KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0093 | - |
2-hydroxy-5-methyl-6-oxohexa-2,4-dienoate | in 100 mM Tris-HCl, pH 8.5, at 25°C | Pseudomonas putida | |
0.017 | - |
2-hydroxymuconate-6-semialdehyde | in 100 mM Tris-HCl, pH 8.5, at 25°C | Pseudomonas putida | |
0.17 | - |
3-Fluorobenzaldehyde | in 100 mM glycine-NaOH (pH 9.2), at 25°C | Pseudomonas putida | |
0.33 | - |
NAD+ | in the presence of benzaldehyde, 100 mM glycine-NaOH (pH 9.2), at 25°C | Pseudomonas putida | |
0.46 | - |
benzaldehyde | in 100 mM glycine-NaOH (pH 9.2), at 25°C | Pseudomonas putida | |
0.53 | - |
4-methylbenzaldehyde | in 100 mM glycine-NaOH (pH 9.2), at 25°C | Pseudomonas putida | |
0.63 | - |
3-methylbenzaldehyde | in 100 mM glycine-NaOH (pH 9.2), at 25°C | Pseudomonas putida | |
0.81 | - |
3-Nitrobenzaldehyde | in 100 mM glycine-NaOH (pH 9.2), at 25°C | Pseudomonas putida | |
0.94 | - |
3-Methoxybenzaldehyde | in 100 mM glycine-NaOH (pH 9.2), at 25°C | Pseudomonas putida | |
1.3 | - |
3-Chlorobenzaldehyde | in 100 mM glycine-NaOH (pH 9.2), at 25°C | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Pseudomonas putida KT 2240 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate fractionation, TSKgel DEAE-5PW column chromatography, Affigel-Blue affinity chromatography, and TSKgel Phenyl-5PW column chromatography | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-hydroxy-5-methyl-6-oxohexa-2,4-dienoate + NAD+ + H2O | - |
Pseudomonas putida | (2E,4Z)-2-hydroxy-5-methylhexa-2,4-diendioate + NADH + H+ | - |
? | |
2-hydroxymuconate-6-semialdehyde + NAD+ + H2O | - |
Pseudomonas putida | (2Z,4E)-2-hydroxyhexa-2,4-dienedioate + NADH + H+ | - |
? | |
3-chlorobenzaldehyde + NAD+ + H2O | - |
Pseudomonas putida | 3-chlorobenzoate + NADH + H+ | - |
? | |
3-fluorobenzaldehyde + NAD+ + H2O | - |
Pseudomonas putida | 3-fluorobenzoate + NADH + H+ | - |
? | |
3-methoxybenzaldehyde + NAD+ + H2O | - |
Pseudomonas putida | 3-methoxybenzoate + NADH + H+ | - |
? | |
3-methylbenzaldehyde + NAD+ + H2O | - |
Pseudomonas putida | 3-methylbenzoate + NADH + H+ | - |
? | |
3-nitrobenzaldehyde + NAD+ + H2O | - |
Pseudomonas putida | 3-nitrobenzoate + NADH + H+ | - |
? | |
4-methylbenzaldehyde + NAD+ + H2O | - |
Pseudomonas putida | 4-methylbenzoate + NADH + H+ | - |
? | |
benzaldehyde + NAD+ + H2O | weak substrate | Pseudomonas putida | benzoate + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
2-hydroxymuconic semialdehyde dehydrogenase | - |
Pseudomonas putida |
XylG | - |
Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.3 | - |
pH optimum for the oxidation of 2-hydroxymuconic semialdehyde | Pseudomonas putida |
9.6 | - |
pH optimum for the oxidation of benzaldehyde | Pseudomonas putida |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8 | - |
3-Methoxybenzaldehyde | apparent value, in 100 mM glycine-NaOH (pH 9.2), at 25°C | Pseudomonas putida | |
10 | - |
4-methylbenzaldehyde | apparent value, in 100 mM glycine-NaOH (pH 9.2), at 25°C | Pseudomonas putida | |
20 | - |
3-Nitrobenzaldehyde | apparent value, in 100 mM glycine-NaOH (pH 9.2), at 25°C | Pseudomonas putida | |
22 | - |
3-Chlorobenzaldehyde | apparent value, in 100 mM glycine-NaOH (pH 9.2), at 25°C | Pseudomonas putida | |
32 | - |
benzaldehyde | apparent value, in 100 mM glycine-NaOH (pH 9.2), at 25°C | Pseudomonas putida | |
36 | - |
3-methylbenzaldehyde | apparent value, in 100 mM glycine-NaOH (pH 9.2), at 25°C | Pseudomonas putida | |
56 | - |
NAD+ | apparent value, in the presence of benzaldehyde, 100 mM glycine-NaOH (pH 9.2), at 25°C | Pseudomonas putida | |
330 | - |
3-Fluorobenzaldehyde | apparent value, in 100 mM glycine-NaOH (pH 9.2), at 25°C | Pseudomonas putida | |
1600 | - |
2-hydroxymuconate-6-semialdehyde | apparent value, in 100 mM Tris-HCl, pH 8.5, at 25°C | Pseudomonas putida | |
2100 | - |
2-hydroxy-5-methyl-6-oxohexa-2,4-dienoate | apparent value, in 100 mM Tris-HCl, pH 8.5, at 25°C | Pseudomonas putida |