Protein Variants | Comment | Organism |
---|---|---|
A441C | the mutant exhibited almost wild type activity with betaine aldehyde | Spinacia oleracea |
A441F | the mutant shows no activity with betaine aldehyde | Spinacia oleracea |
A441I | the mutant shows no activity with betaine aldehyde | Spinacia oleracea |
A441S | the mutant exhibits slightly reduced activity with betaine aldehyde | Spinacia oleracea |
A441T | the mutant exhibits clearly reduced activity with betaine aldehyde | Spinacia oleracea |
A441V | the mutant shows no activity with betaine aldehyde | Spinacia oleracea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0028 | - |
NAD+ | mutant enzyme A441I, at pH 8.0 and 30°C | Spinacia oleracea | |
0.0064 | - |
NAD+ | mutant enzyme A441V, at pH 8.0 and 30°C | Spinacia oleracea | |
0.014 | - |
NAD+ | mutant enzyme A441C, at pH 8.0 and 30°C | Spinacia oleracea | |
0.018 | - |
NAD+ | mutant enzyme A441F, at pH 8.0 and 30°C | Spinacia oleracea | |
0.022 | - |
NAD+ | wild type enzyme, at pH 8.0 and 30°C | Spinacia oleracea | |
0.024 | - |
NAD+ | mutant enzyme A441T, at pH 8.0 and 30°C | Spinacia oleracea | |
0.029 | - |
NAD+ | mutant enzyme A441S, at pH 8.0 and 30°C | Spinacia oleracea | |
0.09 | - |
Betaine aldehyde | mutant enzyme A441C, at pH 8.0 and 30°C | Spinacia oleracea | |
0.098 | - |
Betaine aldehyde | wild type enzyme, at pH 8.0 and 30°C | Spinacia oleracea | |
0.119 | - |
Betaine aldehyde | mutant enzyme A441S, at pH 8.0 and 30°C | Spinacia oleracea | |
0.18 | - |
Betaine aldehyde | mutant enzyme A441T, at pH 8.0 and 30°C | Spinacia oleracea | |
0.512 | - |
Betaine aldehyde | mutant enzyme A441V, at pH 8.0 and 30°C | Spinacia oleracea | |
0.605 | - |
Betaine aldehyde | mutant enzyme A441F, at pH 8.0 and 30°C | Spinacia oleracea | |
1.791 | - |
Betaine aldehyde | mutant enzyme A441I, at pH 8.0 and 30°C | Spinacia oleracea |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
betaine aldehyde + NAD+ + H2O | Spinacia oleracea | - |
betaine + NADH + 2 H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Spinacia oleracea | P17202 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
betaine aldehyde + NAD+ + H2O | - |
Spinacia oleracea | betaine + NADH + 2 H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ALD10 | - |
Spinacia oleracea |
BADH | - |
Spinacia oleracea |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.29 | - |
Betaine aldehyde | mutant enzyme A441F, at pH 8.0 and 30°C | Spinacia oleracea | |
0.39 | - |
NAD+ | mutant enzyme A441F, at pH 8.0 and 30°C | Spinacia oleracea | |
0.51 | - |
NAD+ | mutant enzyme A441V, at pH 8.0 and 30°C | Spinacia oleracea | |
0.54 | - |
Betaine aldehyde | mutant enzyme A441V, at pH 8.0 and 30°C | Spinacia oleracea | |
0.68 | - |
NAD+ | mutant enzyme A441I, at pH 8.0 and 30°C | Spinacia oleracea | |
0.74 | - |
Betaine aldehyde | mutant enzyme A441I, at pH 8.0 and 30°C | Spinacia oleracea | |
2.2 | - |
NAD+ | mutant enzyme A441C, at pH 8.0 and 30°C | Spinacia oleracea | |
2.39 | - |
NAD+ | mutant enzyme A441T, at pH 8.0 and 30°C | Spinacia oleracea | |
2.64 | - |
Betaine aldehyde | mutant enzyme A441T, at pH 8.0 and 30°C | Spinacia oleracea | |
2.99 | - |
Betaine aldehyde | mutant enzyme A441C, at pH 8.0 and 30°C | Spinacia oleracea | |
3.27 | - |
NAD+ | mutant enzyme A441S, at pH 8.0 and 30°C | Spinacia oleracea | |
3.29 | - |
Betaine aldehyde | mutant enzyme A441S, at pH 8.0 and 30°C | Spinacia oleracea | |
3.36 | - |
Betaine aldehyde | wild type enzyme, at pH 8.0 and 30°C | Spinacia oleracea | |
4.25 | - |
NAD+ | wild type enzyme, at pH 8.0 and 30°C | Spinacia oleracea |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Spinacia oleracea |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.41 | - |
Betaine aldehyde | mutant enzyme A441I, at pH 8.0 and 30°C | Spinacia oleracea | |
0.48 | - |
Betaine aldehyde | mutant enzyme A441F, at pH 8.0 and 30°C | Spinacia oleracea | |
1.1 | - |
Betaine aldehyde | mutant enzyme A441V, at pH 8.0 and 30°C | Spinacia oleracea | |
15 | - |
Betaine aldehyde | mutant enzyme A441T, at pH 8.0 and 30°C | Spinacia oleracea | |
22 | - |
NAD+ | mutant enzyme A441F, at pH 8.0 and 30°C | Spinacia oleracea | |
28 | - |
Betaine aldehyde | mutant enzyme A441S, at pH 8.0 and 30°C | Spinacia oleracea | |
33 | - |
Betaine aldehyde | mutant enzyme A441C, at pH 8.0 and 30°C | Spinacia oleracea | |
35 | - |
Betaine aldehyde | wild type enzyme, at pH 8.0 and 30°C | Spinacia oleracea | |
80 | - |
NAD+ | mutant enzyme A441V, at pH 8.0 and 30°C | Spinacia oleracea | |
100 | - |
NAD+ | mutant enzyme A441T, at pH 8.0 and 30°C | Spinacia oleracea | |
114 | - |
NAD+ | mutant enzyme A441S, at pH 8.0 and 30°C | Spinacia oleracea | |
179 | - |
NAD+ | mutant enzyme A441C, at pH 8.0 and 30°C | Spinacia oleracea | |
195 | - |
NAD+ | wild type enzyme, at pH 8.0 and 30°C | Spinacia oleracea | |
243 | - |
NAD+ | mutant enzyme A441I, at pH 8.0 and 30°C | Spinacia oleracea |