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Literature summary for 1.2.1.8 extracted from

  • Ayala-Castro, H.G.; Valenzuela-Soto, E.M.; Figueroa-Soto, C.G.; Munoz-Clares, R.A.
    Complex, unusual conformational changes in kidney betaine aldehyde dehydrogenase suggested by chemical modification with disulfiram (2007), Arch. Biochem. Biophys., 468, 167-173.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Disulfiram CAS 97-77-8, reaction of disulfiram with protein thiol groups by formation of a mixed disulfide or formation of an intra-molecular disulfide resulting in conformational changes. Inactivation under pseudo-first order conditions occurs in a time- and dose-dependent manner. In the absence of disulfiram, but in the presence of 2% methanol as DSF vehicle, no changes in enzymatic activities are observed. Using a DSF concentration range 10-30 microM, inactivation kinetics were biphasic with rate constants differing in one order of magnitude, and inactivation partial. The residual activity at infinite time, decreases as the disulfiram concentrations increases, reaching a value near zero at 30 microM disulfiram, whereas the amplitude of the two inactivation phases increases, each one reaching about 50% of initial activity at 30 microM disulfiram. Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
230000
-
for the homotetramer Sus scrofa

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
the protein undergoes several chromatographic and dialysis steps during purification Sus scrofa

Reaction

Reaction Comment Organism Reaction ID
betaine aldehyde + NAD+ + H2O = betaine + NADH + 2 H+ catalyzes the last irreversible step in the synthesis of the osmoprotector glycine betaine Sus scrofa

Renatured (Commentary)

Renatured (Comment) Organism
2-mercaptoethanol, Reactivation of DSF-inactivated enzyme with 10 mM 2-mercaptoethanol Sus scrofa
DTT, Reactivation of disulfiram-inactivated enzyme with 5 mM DTT Sus scrofa
GSH, Reactivation of DSF-inactivated with 10 mM GSH Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
kidney localized in cortex and medulla cells Sus scrofa
-

Subunits

Subunits Comment Organism
homotetramer
-
Sus scrofa

Synonyms

Synonyms Comment Organism
BADH
-
Sus scrofa
betaine aldehyde dehydrogenase
-
Sus scrofa
betaine aldehyde: NAD+ oxidoreductase
-
Sus scrofa
pkBADH
-
Sus scrofa

Cofactor

Cofactor Comment Organism Structure
NAD+ protective effects of NAD+ against inactivation of BADH by disulfiram Sus scrofa
NADP+
-
Sus scrofa