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Literature summary for 1.2.1.8 extracted from

  • Wang, S.; Yao, Q.; Tao, J.; Qiao, Y.; Zhang, Z.
    Co-ordinate expression of glycine betaine synthesis genes linked by the FMDV 2A region in a single open reading frame in Pichia pastoris (2007), Appl. Microbiol. Biotechnol., 77, 891-899.
    View publication on PubMed
Search for more literature for 1.2.1.8

Activating Compound

Activating Compound Comment Organism Structure
methanol used in the assay as activating compound in concentration of 0.5% for 96 h Suaeda salsa

Cloned(Commentary)

Cloned (Comment) Organism
cloned in different strains (A764, A765, A767 and YPIC3) of Pichia pastoris Suaeda salsa

Inhibitors

Inhibitors Comment Organism Structure
NaCl Yeast strains containing the BADH gene from Suaeda salsa were streaked on YPD medium supplemented with 0.5% methanol and different NaCl concentrations (0-1.5 mol/l). Salt tolerance is examined after incubation at 30°C for 3 days. Growth of yeast strains A764, A765, A767 and YPIC3 is severely suppressed containing 0.8 mol/l NaCl and completely inhibited on 1.0 mol/l NaCl without methanol induction. However, induced with 0.5% of methanol, A764, A765 and A767 grow well but YPIC3 is suppressed greatly on YPD medium containing 1.0 mol/l NaCl. A764, A765, and A767 can resist 1.2 mol/l NaCl and can grow a little on 1.5 mol/l NaCl with 0.5% of methanol induction. Suaeda salsa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
betaine aldehyde + NAD+ + H2O Suaeda salsa glycine betaine synthesis; two-step oxidation involving choline monooxygenase and BADH glycine betaine + NADH + H+ recombinant yeasts transformed with the two genes CMO and BADH exhibited higher tolerance to salt, methanol and high temperature stress. ?

Organism

Organism UniProt Comment Textmining
Suaeda salsa Q155V4
-
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf used for isolating RNA Suaeda salsa
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plant
-
 Suaeda salsa
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root used for isolating RNA Suaeda salsa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
19.63
-
 cloned in strain A764 and extracted, incubating at 30°C for 96 h in presence of 0.5% of methanol. Activity was measured spectrophotometrically at 340 nm in 1 ml assay buffer (50 mmol HEPES-KOH (pH 8.0), 1 mmol EDTA, 5 mmol DTT, 1 mmol NAD+, 1 mmol betaine aldehyde) at 25°C, supplemented with 50 microliter protein extract. Activity is calculated using an extinction coefficient of 6.220 M/cm for NADH. One unit of enzyme activity is defined as the amount converting 1 nmol of NAD+ per min Suaeda salsa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
betaine aldehyde + NAD+ + H2O glycine betaine synthesis; two-step oxidation involving choline monooxygenase and BADH Suaeda salsa glycine betaine + NADH + H+ recombinant yeasts transformed with the two genes CMO and BADH exhibited higher tolerance to salt, methanol and high temperature stress. ?

Synonyms

Synonyms Comment Organism
BADH
-
 Suaeda salsa
betaine aldehyde dehydrogenase
-
 Suaeda salsa

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
additional information
-
 results indicate that the tolerance of the recombinant yeasts to high temperature stress is improved remarkably as a result of improving glycine betaine content in the yeasts expressing betaine synthesis genes from Suaeda salsa Suaeda salsa

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Suaeda salsa