Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-tolualdehyde | only the aldehyde forms and not the gem-diol forms of the inhibitor 3-tolualdehyde bind to the enzyme | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0078 | - |
succinate semialdehyde | pH 8.5, 22°C | Escherichia coli | |
0.0446 | - |
NADP+ | pH 8.5, 22°C | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P25526 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
34 | - |
pH 8.5, 22°C | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-carboxybenzaldehyde + NADP+ + H2O | - |
Escherichia coli | 3-carboxybenzoate + NADPH + 2 H+ | - |
? | |
3-nitrobenzaldehyde + NADP+ + H2O | - |
Escherichia coli | 3-nitrobenzoate + NADPH + 2 H+ | - |
? | |
4-carboxybenzaldehyde + NADP+ + H2O | - |
Escherichia coli | 4-carboxybenzoate + NADPH + H+ | - |
? | |
benzaldehyde + NADP+ + H2O | - |
Escherichia coli | benzoate + NADPH + 2 H+ | - |
? | |
additional information | only the aldehyde forms and not the gem-diol forms of the specific substrate succinic semialdehyde , of selected aldehyde substrates, and of the inhibitor 3-tolualdehyde bind to the enzyme | Escherichia coli | ? | - |
? | |
n-butanal + NADP+ + H2O | - |
Escherichia coli | butanoate + NADPH + 2 H+ | - |
? | |
n-hexanal + NADP+ + H2O | - |
Escherichia coli | hexanoate + NADPH + H+ | - |
? | |
n-pentanal + NADP+ + H2O | - |
Escherichia coli | n-pentanoate + NADPH + H+ | - |
? | |
succinate semialdehyde + NADP+ + H2O | - |
Escherichia coli | succinate + NADPH + 2 H+ | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
75% of maximum activity | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | NAD+ acts as cosubstrate, but the reaction rates are more than 20fold lower than those with NADP+ | Escherichia coli | |
NADP+ | NAD+ also acts as cosubstrate, but the reaction rates are more than 20fold lower than those with NADP+ | Escherichia coli |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.08 | - |
pH 8.5, 22°C | Escherichia coli | 3-tolualdehyde |