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Literature summary for 1.2.1.75 extracted from

  • Liu, C.; Wang, Q.; Xian, M.; Ding, Y.; Zhao, G.
    Dissection of malonyl-coenzyme A reductase of Chloroflexus aurantiacus results in enzyme activity improvement (2013), PLoS ONE, 8, e75554.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.2.1.75

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0238
-
 NADPH isolated C-terminal fragment, pH 7.8, 57°C Chloroflexus aurantiacus
0.0417
-
 NADPH pH 7.8, 57°C Chloroflexus aurantiacus

Organism

Organism UniProt Comment Textmining
Chloroflexus aurantiacus Q6QQP7
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
malonyl-CoA + NADPH + H+
-
 Chloroflexus aurantiacus malonate semialdehyde + CoA + NADP+
-
 ?

Synonyms

Synonyms Comment Organism
MCR
-
 Chloroflexus aurantiacus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
7.81
-
 NADPH pH 7.8, 57°C Chloroflexus aurantiacus
18.6
-
 NADPH isolated C-terminal fragment, pH 7.8, 57°C Chloroflexus aurantiacus

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Chloroflexus aurantiacus

General Information

General Information Comment Organism
physiological function the N-terminal region of MCR (MCR-N, amino acids 1-549) and the C-terminal region of MCR (MCR-C, amino acids 550-1219) are functionally distinct. Malonyl-CoA is reduced into free intermediate malonate semialdehyde with NADPH by the MCR-C fragment, and further reduced to 3-hydroxypropionate by the MCR-N fragment, the initial reduction of malonyl-CoA being rate limiting. The TGXXXG(A)X(1-2)G and YXXXK motifs are important for enzyme activities of both MCR-N and MCR-C fragments, and the enzyme activity increases when MCR is separated into two individual fragments. The MCR-C fragment has higher affinity for malonyl-CoA and 4-times higher Kcat/Km value than MCR Chloroflexus aurantiacus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
187
-
 NADPH pH 7.8, 57°C Chloroflexus aurantiacus
782
-
 NADPH isolated C-terminal fragment, pH 7.8, 57°C Chloroflexus aurantiacus