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Literature summary for 1.2.1.75 extracted from

  • Kildegaard, K.; Jensen, N.; Schneider, K.; Czarnotta, E.; Özdemir, E.; Klein, T.; Maury, J.; Ebert, B.; Christensen, H.; Chen, Y.; Kim, I.; Herrgard, M.; Blank, L.; Forster, J.; Nielsen, J.; Borodina, I.
    Engineering and systems-level analysis of Saccharomyces cerevisiae for production of 3-hydroxypropionic acid via malonyl-CoA reductase-dependent pathway (2016), Microb. Cell Fact., 15, 53.
    View publication on PubMedView publication on EuropePMC
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Application

Application Comment Organism
synthesis integration of multiple copies of malonyl-CoA reductase MCR and of phosphorylation-deficient acetyl-CoA carboxylase ACC1 genes into the genome of yeast increases 3-hydroxypropionic acid titer fivefold in comparison with single integration. Optimizing the supply of acetyl-CoA by overexpressing native pyruvate decarboxylase PDC1, aldehyde dehydrogenase ALD6, and acetyl-CoA synthase from Salmonella enterica SEacsL641P engineering the cofactor specificity of the glyceraldehyde-3-phosphate dehydrogenase to increase the intracellular production of NADPH at the expense of NADH improves 3-hydroxypropionic acid production and reduces formation of glycerol as by-product. The final strain produces 9.8 g per L 3-hydroxypropionic acid with a yield of 13% C-mol per C-mol glucose after 100 h in carbon-limited fed-batch cultivation at pH 5 Chloroflexus aurantiacus

Organism

Organism UniProt Comment Textmining
Chloroflexus aurantiacus Q6QQP7
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