Activating Compound | Comment | Organism | Structure |
---|---|---|---|
glutamate-1-semialdehyde aminotransferase | 2.5fold activity in presence of glutamate-1-semialdehyde aminotransferase | Chlamydomonas reinhardtii |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Chlamydomonas reinhardtii |
Protein Variants | Comment | Organism |
---|---|---|
C48A | no activity | Chlamydomonas reinhardtii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46000 | - |
2 * 52500 + 2 * 46000, sucrose gradient sedimentation of glutamyl-tRNA reductase preincubated with glutamate-1-semialdehyde aminotransferase. Both enzyme also co-precipitate in immunoprecipitation experiments | Chlamydomonas reinhardtii |
52500 | - |
2 * 52500 + 2 * 46000, sucrose gradient sedimentation of glutamyl-tRNA reductase preincubated with glutamate-1-semialdehyde aminotransferase. Both enzyme also co-precipitate in immunoprecipitation experiments | Chlamydomonas reinhardtii |
52500 | - |
2 * 52500, sucrose gradient sedimentation compared to calculation from sequence | Chlamydomonas reinhardtii |
101000 | - |
sucrose density gradient sedimentation, dimer | Chlamydomonas reinhardtii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chlamydomonas reinhardtii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-affinity chromatography | Chlamydomonas reinhardtii |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Chlamydomonas reinhardtii | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamyl-tRNAGlu + NADH + H+ | - |
Chlamydomonas reinhardtii | L-glutamate 1-semialdehyde + NAD+ + tRNAGlu | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 52500, sucrose gradient sedimentation compared to calculation from sequence | Chlamydomonas reinhardtii |
heterotetramer | 2 * 52500 + 2 * 46000, sucrose gradient sedimentation of glutamyl-tRNA reductase preincubated with glutamate-1-semialdehyde aminotransferase. Both enzyme also co-precipitate in immunoprecipitation experiments | Chlamydomonas reinhardtii |
Synonyms | Comment | Organism |
---|---|---|
glutamyl-tRNA reductase | - |
Chlamydomonas reinhardtii |
GTR | - |
Chlamydomonas reinhardtii |