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Literature summary for 1.2.1.70 extracted from

  • Vothknecht, U.C.; Kannangara, C.G.; von Wettstein, D.
    Expression of catalytically active barley glutamyl tRNAGlu reductase in Escherichia coli as a fusion protein with glutathione S-transferase (1996), Proc. Natl. Acad. Sci. USA, 93, 9287-9291.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.2.1.70

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli as a fusion protein with glutathione S-transferase Hordeum vulgare

Inhibitors

Inhibitors Comment Organism Structure
hemin 50% inhibition at 0.0015 mM Hordeum vulgare

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 4 * 60000, SDS-PAGE Hordeum vulgare
250000
-
 gel filtration Hordeum vulgare

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamyl-tRNAGlu + NADPH + H+ Hordeum vulgare the enzyme catalyzes the initial step of tetrapyrrole biosynthesis L-glutamate 1-semialdehyde + NADP+ + tRNAGlu
-
 ?

Organism

Organism UniProt Comment Textmining
Hordeum vulgare Q42843
-
-

Purification (Commentary)

Purification (Comment) Organism
fusion protein with glutathione S-transferase Hordeum vulgare

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.12
-
 fusion protein with glutathione S-transferase Hordeum vulgare

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamyl-tRNAGlu + NADPH + H+ the enzyme catalyzes the initial step of tetrapyrrole biosynthesis Hordeum vulgare L-glutamate 1-semialdehyde + NADP+ + tRNAGlu
-
 ?
L-glutamyl-tRNAGlu + NADPH + H+ the fusion protein with glutathione S-transferase uses tRNAGlu from Hordeum vulgare chloroplast preferentially to Escherichia coli tRNAGlu Hordeum vulgare L-glutamate 1-semialdehyde + NADP+ + tRNAGlu
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 60000, SDS-PAGE Hordeum vulgare

Cofactor

Cofactor Comment Organism Structure
heme the fusion protein with glutathione S-transferase contains heme, which can be reduced by NADPH and oxidized by air Hordeum vulgare
NADPH
-
 Hordeum vulgare