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Literature summary for 1.2.1.68 extracted from

  • Overhage, J.; Steinbuchel, A.; Priefert, H.
    Biotransformation of eugenol to ferulic acid by a recombinant strain of Ralstonia eutropha H16 (2002), Appl. Environ. Microbiol., 68, 4315-4321.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
biotechnology biotransformation of eugenol to ferulic acid by a recombinant strain of Ralstonia eutropha H16. The gene calB, encoding coniferyl aldehyde dehydrogenase, and ehyAB and calA encoding eugenol hydroxylase and coniferyl alcohol dehydrogenase, respectively, are amplified and combined to construct a catabolic gene cassette. This gene cassette is cloned in the broad-host-range vector pBBR1-JO2 and transferred to Ralstonia eutropha H16. A recombinant strain of Ralstonia eutropha H16 harboring this plasmid expresses functionally active eugenol hydroxylase, coniferyl alcohol dehydrogenase, and coniferyl aldehyde dehydrogenase. Cells of Ralstonia eutropha H16 from the late-exponential growth phase are used asa biocatalysts for the biotransformation of eugenol to ferulic acid. A maximum conversion rate of 2.9 mM of eugenol per h per liter of culture is achieved with a yield of 93.8 mol% of ferulic acid from eugenol within 20 h, without further optimization Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
coniferyl aldehyde + H2O + NAD+ Pseudomonas sp. involved in eugenol catabolism ferulate + NADH
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Organism

Organism UniProt Comment Textmining
Pseudomonas sp. Q86447
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
coniferyl aldehyde + H2O + NAD+
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Pseudomonas sp. ferulate + NADH
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coniferyl aldehyde + H2O + NAD+ involved in eugenol catabolism Pseudomonas sp. ferulate + NADH
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Synonyms

Synonyms Comment Organism
coniferyl aldehyde dehydrogenase
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Pseudomonas sp.