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Literature summary for 1.2.1.59 extracted from
- Purification and characterization of D-glyceraldehyde-3-phosphate dehydrogenase from the thermophilic archaebacterium Methanothermus fervidus (1987), Eur. J. Biochem., 165, 147-155.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Methanothermus fervidus |
General Stability
| General Stability | Organism |
|---|---|
| NADP+ stabilizes against thermal inactivation, NAD+ has no effect | Methanothermus fervidus |
| salts stabilize against thermal inactivation in the order: potassium phosphate > sodium phosphate > K2SO4 > sodium citrate > KCl > NaCl | Methanothermus fervidus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | insensitive to pentalenolactone | Methanothermus fervidus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.002 | - |
NADPH | - |
Methanothermus fervidus |  |
| 0.003 | - |
NADP+ | - |
Methanothermus fervidus | |
| 0.066 | - |
NADPH | - |
Methanothermus fervidus | |
| 0.65 | - |
NAD+ | - |
Methanothermus fervidus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| phosphate | dependent on | Methanothermus fervidus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
4 * 45000, SDS-PAGE | Methanothermus fervidus |
| 200000 | - |
gel filtration | Methanothermus fervidus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanothermus fervidus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Methanothermus fervidus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 49.59 | - |
- |
Methanothermus fervidus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Methanothermus fervidus | 3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
r | |
| D-glyceraldehyde-3-phosphate + phosphate + NAD(P)+ | r | Methanothermus fervidus | 1,3-diphosphoglycerate + NAD(P)H | - |
? | |
| D-glyceraldehyde-3-phosphate + phosphate + NADP+ | r | Methanothermus fervidus | 1,3-diphosphoglycerate + NADPH | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 45000, SDS-PAGE | Methanothermus fervidus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
pH 7.0, 30 min, stable | Methanothermus fervidus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.4 | - |
- |
Methanothermus fervidus |
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