Cloned (Comment) | Organism |
---|---|
expression at high levels in Escherichia coli, the primary structure exhibits a strikingly high proportion of aromatic amino acids | Pyrococcus woesei |
General Stability | Organism |
---|---|
several salts stabilize against thermal inactivation, K+-citrate is most effective | Pyrococcus woesei |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | insensitive to pentalenolactone | Pyrococcus woesei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
NADP+ | - |
Pyrococcus woesei | |
1 | - |
NAD+ | - |
Pyrococcus woesei |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
phosphate | dependent on | Pyrococcus woesei | |
phosphate | Km: 0.04 mM | Pyrococcus woesei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus woesei | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pyrococcus woesei |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Pyrococcus woesei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Pyrococcus woesei | 3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
r | |
D-glyceraldehyde-3-phosphate + phosphate + NAD(P)+ | - |
Pyrococcus woesei | 1,3-diphosphoglycerate + NAD(P)H | - |
? | |
D-glyceraldehyde-3-phosphate + phosphate + NADP+ | - |
Pyrococcus woesei | 1,3-diphosphoglycerate + NADPH | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
100 | - |
half-life: 44 min | Pyrococcus woesei |