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Literature summary for 1.2.1.50 extracted from
- Fatty acid reductase from Photobacterium phosphoreum (1986), Methods Enzymol., 133, 172-182.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| requires thiol reducing agents for stability and activity | Photobacterium phosphoreum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.001 | - |
tetradecanoyl-CoA | - |
Photobacterium phosphoreum |  |
| 0.005 | - |
NADPH | - |
Photobacterium phosphoreum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34000 | - |
x * 58000 + x * 34000 + x * 50000, reductase, transferase and synthetase subunit of the enzyme complex, SDS-PAGE | Photobacterium phosphoreum |
| 50000 | - |
x * 58000 + x * 34000 + x * 50000, reductase, transferase and synthetase subunit of the enzyme complex, SDS-PAGE | Photobacterium phosphoreum |
| 58000 | - |
x * 58000 + x * 34000 + x * 50000, reductase, transferase and synthetase subunit of the enzyme complex, SDS-PAGE | Photobacterium phosphoreum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| tetradecanoyl-CoA + NADPH + H+ | Photobacterium phosphoreum | - |
tetradecanal + CoA + NADP+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Photobacterium phosphoreum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Photobacterium phosphoreum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 25 | - |
- |
Photobacterium phosphoreum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 50 mM phosphate buffer, pH 7.0, 0.2 M NaCl, 50 mM beta-mercaptoethanol, 30% glycerol, several months | Photobacterium phosphoreum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| tetradecanoyl-CoA + NADPH + H+ | - |
Photobacterium phosphoreum | tetradecanal + CoA + NADP+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| polymer | x * 58000 + x * 34000 + x * 50000, reductase, transferase and synthetase subunit of the enzyme complex, SDS-PAGE | Photobacterium phosphoreum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | 3% of NADPH activity | Photobacterium phosphoreum | |
| NADPH | - |
Photobacterium phosphoreum | |
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