Activating Compound | Comment | Organism | Structure |
---|---|---|---|
D,L-2-amino-1-propanol | activation of aldehyde oxidation | Rattus norvegicus | |
L-serine methyl ester | activation of aldehyde oxidation | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.14 | - |
methylglyoxal | - |
Rattus norvegicus | |
0.21 | 0.83 | methylglyoxal | - |
Rattus norvegicus | |
0.8 | - |
NADP+ | - |
Rattus norvegicus | |
8 | - |
methylglyoxal | amine as activator in aldehyde oxidation | Rattus norvegicus | |
8 | - |
methylglyoxal | in the presence of L-serine methyl ester (Km 8.0 mM) | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
gel filtration | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
methylglyoxal + NADP+ + H2O | Rattus norvegicus | ir | pyruvate + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using ammonium sulfate fractionation and chromatography on DEAE-Sephadex column, Blue-dextran affinity column and CM-Sephadex column | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Rattus norvegicus |
7.25 | - |
- |
Rattus norvegicus |
Storage Stability | Organism |
---|---|
-20°C, 50% glycerol | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methylglyoxal + NADP+ + H2O | ir | Rattus norvegicus | pyruvate + NADPH + H+ | - |
? | |
additional information | the only known amine which activates the enzyme at physiological pH is L-serine methyl ester, L-2-amino-1-propanol gives very little activity but D,L-2-amino-1-propanol activates the enzyme | Rattus norvegicus | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
9.3 | - |
activity falls abruptly above | Rattus norvegicus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
9.3 | - |
not stable above | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Rattus norvegicus |