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Literature summary for 1.2.1.49 extracted from

  • Dunkerton, J.; James, S.P.
    Some observations on the NADP+-linked oxidation of methylglyoxal catalysed by 2-Oxoaldehyde dehydrogenase (1976), Biochem. J., 153, 503-504.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
L-2-Aminopropan-1-ol activation of aldehyde oxidation Ovis aries

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0045
-
NADP+
-
Ovis aries
0.21 0.83 methylglyoxal
-
Ovis aries
0.83
-
methylglyoxal
-
Ovis aries
0.83
-
methylglyoxal amine as activator in aldehyde oxidation Ovis aries
0.83
-
methylglyoxal in the presence of L-2-aminopropan-1-ol (Km 83 mM), at pH 9.3 and 25°C Ovis aries

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
methylglyoxal + NADP+ + H2O Ovis aries
-
pyruvate + NADPH + H+
-
?

Organism

Organism UniProt Comment Textmining
Ovis aries
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Ovis aries
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.24
-
-
Ovis aries

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoaldehydes + NADP+ + H2O enzyme from sheep or rat liver also active with NAD+, see also EC 1.2.1.23 Ovis aries 2-oxo acids + NADPH
-
?
methylglyoxal + NADP+ + H2O
-
Ovis aries pyruvate + NADPH + H+
-
?
additional information the presence of L-2-aminopropan-1-ol is necessary to activate the enzyme Ovis aries ?
-
?

Cofactor

Cofactor Comment Organism Structure
NAD+ enzyme from sheep or rat liver, see also EC 1.2.1.23 Ovis aries
NADP+
-
Ovis aries