Activating Compound | Comment | Organism | Structure |
---|---|---|---|
alkane | transcriptional induction of Bt-aldh gene by alkane, Bt-aldh dramatically increases after 10 day cultivation, exactly when the alkane degradation starts | Geobacillus thermoleovorans |
Cloned (Comment) | Organism |
---|---|
expression and overproduction in Escherichia coli | Geobacillus thermoleovorans |
expression in Escherichia coli | Geobacillus thermoleovorans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 2 mM, 63% residual activity; 63% relative activity | Geobacillus thermoleovorans | |
Mg2+ | 14% relative activity with 2 mM MgCl2 compared to the activity without metal ions; 2 mM, 14% residual activity | Geobacillus thermoleovorans | |
Zn2+ | 2 mM, 0.1% residual activity; relative activity is above 1% with 2 mM ZnCl2 compared to the activity without metal ions | Geobacillus thermoleovorans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | activity is elevated 2fold in the presence of 2 mM | Geobacillus thermoleovorans | |
Ba2+ | 2 mM, 2-3fold stimulation | Geobacillus thermoleovorans | |
Ca2+ | activity is elevated 2fold in the presence of 2 mM | Geobacillus thermoleovorans | |
Ca2+ | 2 mM, 2-3fold stimulation | Geobacillus thermoleovorans | |
Sr2+ | activity is elevated 3fold in the presence of 2 mM | Geobacillus thermoleovorans | |
Sr2+ | 2 mM, 2-3fold stimulation | Geobacillus thermoleovorans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53886 | - |
8 * 53886, calculated, 8 * 55000, SDS-PAGE | Geobacillus thermoleovorans |
53890 | - |
monomer is calculated from the 497 amino acid residues | Geobacillus thermoleovorans |
55000 | - |
8 * 55000, determined by SDS-PAGE | Geobacillus thermoleovorans |
55000 | - |
8 * 53886, calculated, 8 * 55000, SDS-PAGE | Geobacillus thermoleovorans |
410000 | - |
gel filtration | Geobacillus thermoleovorans |
410000 | - |
estimated by gel filtration | Geobacillus thermoleovorans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetaldehyde + NAD+ + H2O | Geobacillus thermoleovorans | - |
acetate + NADH + H+ | - |
? | |
acetaldehyde + NAD+ + H2O | Geobacillus thermoleovorans B23 | - |
acetate + NADH + H+ | - |
? | |
benzaldehyde + NAD+ + H2O | Geobacillus thermoleovorans | - |
benzoate + NADH + H+ | - |
? | |
benzaldehyde + NAD+ + H2O | Geobacillus thermoleovorans B23 | - |
benzoate + NADH + H+ | - |
? | |
betaine aldehyde + NAD+ + H2O | Geobacillus thermoleovorans | - |
? | - |
? | |
glyceraldehyde + NAD+ + H2O | Geobacillus thermoleovorans | - |
? | - |
? | |
hexanal + NAD+ + H2O | Geobacillus thermoleovorans | - |
? | - |
? | |
additional information | Geobacillus thermoleovorans | enzyme has high activity against aliphatic long-chain aldehydes such as tetradecanal, Bt-Aldh represents thermophilic aldehyde dehydrogenases responsible for degradation of long-chain alkanes | ? | - |
? | |
additional information | Geobacillus thermoleovorans B23 | enzyme has high activity against aliphatic long-chain aldehydes such as tetradecanal, Bt-Aldh represents thermophilic aldehyde dehydrogenases responsible for degradation of long-chain alkanes | ? | - |
? | |
octanal + NAD+ + H2O | Geobacillus thermoleovorans | - |
octanoate + NADH + H+ | - |
? | |
octanal + NAD+ + H2O | Geobacillus thermoleovorans B23 | - |
octanoate + NADH + H+ | - |
? | |
p-cumic aldehyde + NAD+ + H2O | Geobacillus thermoleovorans | - |
? | - |
? | |
retinal + NAD+ + H2O | Geobacillus thermoleovorans | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus thermoleovorans | Q9FAB1 | - |
- |
Geobacillus thermoleovorans B23 | Q9FAB1 | - |
- |
Purification (Comment) | Organism |
---|---|
crude enzyme solution is applied to an anionic exchange column and eluted by linearly increasing the NaCl concentration from 0-1.0 M in 50 mM Tris-HCl at pH 7.0 | Geobacillus thermoleovorans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
110% relative activity with 2 mM MnCl2 compared to the activity without metal ions, pH 10, 50 mM glycine buffer, 37°C, 1 mM hexanal, 1 mM NAD+ | Geobacillus thermoleovorans |
additional information | - |
120% relative activity with 2 mM CoCl2 compared to the activity without metal ions, pH 10, 50 mM glycine buffer, 37°C, 1 mM hexanal, 1 mM NAD+ | Geobacillus thermoleovorans |
additional information | - |
14% relative activity with 2 mM MgCl2 compared to the activity without metal ions, pH 10, 50 mM glycine buffer, 37°C, 1 mM hexanal, 1 mM NAD+ | Geobacillus thermoleovorans |
additional information | - |
190% relative activity with 2 mM CaCl2 EDTA compared to the activity without metal ions, pH 10, 50 mM glycine buffer, 37°C, 1 mM hexanal, 1 mM NAD+ | Geobacillus thermoleovorans |
additional information | - |
210% relative activity with 2 mM BaCl2 compared to the activity without metal ions, pH 10, 50 mM glycine buffer, 37°C, 1 mM hexanal, 1 mM NAD+ | Geobacillus thermoleovorans |
additional information | - |
340% relative activity with 2 mM SrCl2 compared to the activity without metal ions, pH 10, 50 mM glycine buffer, 37°C, 1 mM hexanal, 1 mM NAD+ | Geobacillus thermoleovorans |
additional information | - |
63% relative activity with 2 mM EDTA compared to the activity without metal ions, pH 10, 50 mM glycine buffer, 37°C, 1 mM hexanal, 1 mM NAD+ | Geobacillus thermoleovorans |
additional information | - |
87% relative activity with 2 mM CuCl2 compared to the activity without metal ions, pH 10, 50 mM glycine buffer, 37°C, 1 mM hexanal, 1 mM NAD+ | Geobacillus thermoleovorans |
additional information | - |
97% relative activity with 2 mM NiCl2 compared to the activity without metal ions, pH 10, 50 mM glycine buffer, 37°C, 1 mM hexanal, 1 mM NAD+ | Geobacillus thermoleovorans |
additional information | - |
in the range of substrate aldehydes with carbon numbers C2 to C14, Bt-Aldh is the most active against C14, tetradecanal at 55°C, the activity against tetradecanal is 73times higher than that against octanal | Geobacillus thermoleovorans |
additional information | - |
relative activity is above 1% with 2 mM ZnCl2 compared to the activity without metal ions, pH 10, 50 mM glycine buffer, 37°C, 1 mM hexanal, 1 mM NAD+ | Geobacillus thermoleovorans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetaldehyde + NAD+ + H2O | - |
Geobacillus thermoleovorans | acetate + NADH + H+ | - |
? | |
acetaldehyde + NAD+ + H2O | - |
Geobacillus thermoleovorans B23 | acetate + NADH + H+ | - |
? | |
benzaldehyde + NAD+ + H2O | - |
Geobacillus thermoleovorans | benzoate + NADH + H+ | - |
? | |
benzaldehyde + NAD+ + H2O | - |
Geobacillus thermoleovorans B23 | benzoate + NADH + H+ | - |
? | |
betaine aldehyde + NAD+ + H2O | - |
Geobacillus thermoleovorans | ? | - |
? | |
dodecanal + NAD+ + H2O | 50% of the activity with tetradecanal | Geobacillus thermoleovorans | dodecanoic acid + NADH + H+ | - |
? | |
glyceraldehyde + NAD+ + H2O | - |
Geobacillus thermoleovorans | ? | - |
? | |
hexanal + NAD+ + H2O | - |
Geobacillus thermoleovorans | ? | - |
? | |
hexanal + NAD+ + H2O | 50% of the activity with tetradecanal | Geobacillus thermoleovorans | hexanoic acid + NADH + H+ | - |
? | |
additional information | enzyme has high activity against aliphatic long-chain aldehydes such as tetradecanal, Bt-Aldh represents thermophilic aldehyde dehydrogenases responsible for degradation of long-chain alkanes | Geobacillus thermoleovorans | ? | - |
? | |
additional information | enzyme displays high activity against aliphatic long-chain aldehydes such as tetradecanal | Geobacillus thermoleovorans | ? | - |
? | |
additional information | enzyme has high activity against aliphatic long-chain aldehydes such as tetradecanal, Bt-Aldh represents thermophilic aldehyde dehydrogenases responsible for degradation of long-chain alkanes | Geobacillus thermoleovorans B23 | ? | - |
? | |
additional information | enzyme displays high activity against aliphatic long-chain aldehydes such as tetradecanal | Geobacillus thermoleovorans B23 | ? | - |
? | |
octanal + NAD+ + H2O | - |
Geobacillus thermoleovorans | octanoate + NADH + H+ | - |
? | |
octanal + NAD+ + H2O | - |
Geobacillus thermoleovorans B23 | octanoate + NADH + H+ | - |
? | |
octanal + NAD+ + H2O | 50% of the activity with tetradecanal | Geobacillus thermoleovorans | octanoic acid + NADH + H+ | - |
? | |
p-cumic aldehyde + NAD+ + H2O | - |
Geobacillus thermoleovorans | ? | - |
? | |
retinal + NAD+ + H2O | - |
Geobacillus thermoleovorans | ? | - |
? | |
tetradecanal + NAD+ + H2O | - |
Geobacillus thermoleovorans | tetradecanoic acid + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
octamer | 8 * 55000, determined by SDS-PAGE | Geobacillus thermoleovorans |
octamer | 8 * 53886, calculated, 8 * 55000, SDS-PAGE | Geobacillus thermoleovorans |
Synonyms | Comment | Organism |
---|---|---|
Bt-Aldh | - |
Geobacillus thermoleovorans |
long-chain-aldehyde dehydrogenase | - |
Geobacillus thermoleovorans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 55 | - |
Geobacillus thermoleovorans |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 55 | optimum condition for activity | Geobacillus thermoleovorans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
stable up to | Geobacillus thermoleovorans |
70 | - |
activity decreases to 12.7% after a heat treatment at 70°C for 30 min | Geobacillus thermoleovorans |
70 | - |
30 min, 12.7% residual activity | Geobacillus thermoleovorans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10 | - |
- |
Geobacillus thermoleovorans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | activity of Bt-Aldh is doubled in the presence of NAD+ than NADP+ | Geobacillus thermoleovorans | |
NADH | - |
Geobacillus thermoleovorans | |
NADP+ | - |
Geobacillus thermoleovorans | |
NADPH | about 50% of the activity with NADH | Geobacillus thermoleovorans |
Organism | Comment | Expression |
---|---|---|
Geobacillus thermoleovorans | expression level is dramatically increased when alkane degradation is started | up |