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Literature summary for 1.2.1.48 extracted from

  • Kelson, T.L.; Secor McVoy, J.R.; Rizzo, W.B.
    Human liver fatty aldehyde dehydrogenase: microsomal localization, purification, and biochemical characterization (1997), Biochim. Biophys. Acta, 1335, 99-110.
    View publication on PubMed
Search for more literature for 1.2.1.48

Inhibitors

Inhibitors Comment Organism Structure
alpha,p-dibromoacetophenone 0.03 mM causes 100% inhibition Homo sapiens
Chloral hydrate 25 mM causes 29% inhibition Homo sapiens
Disulfiram 0.065 mM causes 100% inhibition Homo sapiens
iodoacetamide 10 mM causes 96% inhibition Homo sapiens
iodoacetate 10 mM causes 92% inhibition Homo sapiens
N-ethylmaleimide 2 mM causes 43% inhibition Homo sapiens
p-chloromercuribenzoate 0.01 mM causes complete inhibition Homo sapiens
p-hydroxyacetophenone 0.25 mM causes 46% inhibition Homo sapiens
potassium cyanide 1 mM causes 33% inhibition Homo sapiens
Zn2+ 1 mM causes 47% inhibition Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.006
-
 cis,cis-9,12-octadecadienal
-
 Homo sapiens
0.006
-
 dihydrophytal
-
 Homo sapiens
0.011
-
 cis-9-octadecenal
-
 Homo sapiens
0.012
-
 cis-9-hexadecenal
-
 Homo sapiens
0.018
-
 tetraeicosanal
-
 Homo sapiens
0.019
-
 dodecanal
-
 Homo sapiens
0.021
-
 octadecanal
-
 Homo sapiens
0.023
-
 decanal
-
 Homo sapiens
0.023
-
 Tetradecanal
-
 Homo sapiens
0.028
-
 Pentadecanal
-
 Homo sapiens
0.032
-
 octanal
-
 Homo sapiens
0.032
-
 hexadecanal
-
 Homo sapiens
0.036
-
 behenic aldehyde
-
 Homo sapiens
0.038
-
 arachidic aldehyde
-
 Homo sapiens
0.05
-
 hexanal
-
 Homo sapiens
0.28
-
 NAD+ reference substrate octadecanal Homo sapiens
0.8
-
 crotonaldehyde
-
 Homo sapiens
1.3
-
 propionaldehyde
-
 Homo sapiens
1.44
-
 benzaldehyde
-
 Homo sapiens
1.7
-
 Glutaraldehyde
-
 Homo sapiens
2.5
-
 acetaldehyde
-
 Homo sapiens
8.7
-
 NADP+ reference substrate octadecanal Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Homo sapiens
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54000
-
 x * 54000, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
long-chain aldehyde + NAD+ Homo sapiens
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using chromatography on columns consisting of omega-aminohexyl-agarose and 5'-AMP-Sepharose 4B Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
33.3
-
-
 Homo sapiens

Storage Stability

Storage Stability Organism
-20°C, 25 mM potassium phosphate, pH 7.5, 5% glycerol, 0.2 mM NAD+, 3 months, retains more than 80% of its original activity Homo sapiens
4°C, 25 mM potassium phosphate, pH 7.5, 5% glycerol, 0.2 mM NAD+, 1 month, retains 67% of its original activity Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetaldehyde + NAD+ + H2O
-
 Homo sapiens acetate + NADH + H+
-
 ?
arachidic aldehyde + NAD+ + H2O
-
 Homo sapiens arachidic acid + NADH
-
 ?
behenic aldehyde + NAD+ + H2O
-
 Homo sapiens behenic acid + NADH
-
 ?
benzaldehyde + NAD+ + H2O
-
 Homo sapiens benzoate + NADH + H+
-
 ?
cis,cis-9,12-octadecadienal + NAD+ + H2O
-
 Homo sapiens cis,cis-9,12-octadecadienoic acid + NADH
-
 ?
cis-9-hexadecenal + NAD+ + H2O
-
 Homo sapiens cis-9-hexadecenoic acid + NADH
-
 ?
cis-9-octadecenal + NAD+ + H2O
-
 Homo sapiens cis-9-octadecenoic acid + NADH
-
 ?
crotonaldehyde + NAD+ + H2O
-
 Homo sapiens crotonic acid + NADH
-
 ?
decanal + NAD+ + H2O
-
 Homo sapiens decanoic acid + NADH
-
 ?
dihydrophytal + NAD+ + H2O
-
 Homo sapiens (3R,S,7R,11R)-3,7,11,15-tetramethylhexadecanoic acid + NADH
-
 ?
dodecanal + NAD+ + H2O
-
 Homo sapiens dodecanoic acid + NADH
-
 ?
glutaraldehyde + NAD+
-
 Homo sapiens glutaric acid + NADH
-
 ?
hexadecanal + NAD+ + H2O
-
 Homo sapiens hexadecanoic acid + NADH
-
 ?
hexanal + NAD+ + H2O
-
 Homo sapiens hexanoate + NADH + H+
-
 ?
long-chain aldehyde + NAD+
-
 Homo sapiens ?
-
 ?
long-chain aldehyde + NAD+ + H2O
-
 Homo sapiens long-chain acid anion + NADH
-
 ?
additional information poor substrates: acetaldehyde, propionaldehyde, crotonaldehyde, glutaraldehyde, benzaldehyde, retinaldehyde Homo sapiens ?
-
 ?
octadecanal + NAD+ + H2O
-
 Homo sapiens octadecanoic acid + NADH
-
 ?
octadecanal + NADP+
-
 Homo sapiens octadecanoic acid + NADPH + H+
-
 r
octanal + NAD+ + H2O
-
 Homo sapiens octanoic acid + NADH + H+
-
 ?
pentadecanal + NAD+ + H2O
-
 Homo sapiens pentadecanoic acid + NADH
-
 ?
propionaldehyde + NAD+ + H2O
-
 Homo sapiens propionic acid + NADH
-
 ?
tetradecanal + NAD+ + H2O
-
 Homo sapiens tetradecanoic acid + NADH
-
 ?
tetraeicosanal + NAD+ + H2O
-
 Homo sapiens tetraeicosanoic acid + NADH
-
 ?

Subunits

Subunits Comment Organism
? x * 54000, SDS-PAGE Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
47
-
 5 min, the purified enzyme retains only 13% of its original activity Homo sapiens
52
-
 5 min, 100% loss of activity Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.8
-
 in glycine buffer Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NAD+ the enzyme has a much greater specificity for NAD+ as cofactor than NADP+ Homo sapiens