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Literature summary for 1.2.1.46 extracted from

  • Demkiv, O.; Smutok, O.; Paryzhak, S.; Gayda, G.; Sultanov, Y.; Guschin, D.; Shkil, H.; Schuhmann, W.; Gonchar, M.
    Reagentless amperometric formaldehyde-selective biosensors based on the recombinant yeast formaldehyde dehydrogenase (2008), Talanta, 76, 837-846.
    View publication on PubMed

Application

Application Comment Organism
biotechnology development of novel formaldehyde-selective amperometric biosensors based on immobilized NAD+- and glutathione dependent formaldehyde dehydrogenase with high selectivity to formaldehyde and a low cross-sensitivity to other substances, the laboratory prototype of the sensor is applied for FA testing in some real samples of pharmaceutical (formidron), disinfectant (descoton forte) and industrial product (formalin) Ogataea angusta

Cloned(Commentary)

Cloned (Comment) Organism
construction of a recombinant yeast clone originated from the recipient strain of thermotolerant methylotrophic yeast Hansenula polymorpha which is a NAD+- and glutathione dependent FdDH over-producer Ogataea angusta

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.18
-
formaldehyde KM value of the used FdDH in solution from the wild strains Ogataea angusta
119.9
-
formaldehyde immobilized enzyme in a bioactive layer on the transducer surface, apparent Michaelis-Menten constant derived from the formaldehyde calibration curves is 120 mM with a linear detection range for formaldehyde up to 20 mM Ogataea angusta

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
formaldehyde + NAD+ + H2O Ogataea angusta key enzyme of formaldehyde metabolism in microorganisms formate + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Ogataea angusta
-
-
-

Purification (Commentary)

Purification (Comment) Organism
FdDH ss isolated from the cell-free extract by a two-step ion exchange chromatography Ogataea angusta

Storage Stability

Storage Stability Organism
14 h and 1 mM during 22 h, the storage stability of the developed biosensors is found to be longer than 18 days at 4°C Ogataea angusta
the developed sensors demonstrate a good operational stability at comparatively low formaldehyde concentrations of 2 mM during Ogataea angusta

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
formaldehyde + NAD+ + H2O key enzyme of formaldehyde metabolism in microorganisms Ogataea angusta formate + NADH + H+
-
?

Synonyms

Synonyms Comment Organism
FdDH
-
Ogataea angusta
formaldehyde dehydrogenase
-
Ogataea angusta
NAD+-dependent formaldehyde dehydrogenase
-
Ogataea angusta

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
45 50 optimal temperature for the developed FdDH-based biosensor Ogataea angusta

pH Range

pH Minimum pH Maximum Comment Organism
7.6 8.3 optimal pH-value for the developed FdDH-based biosensor Ogataea angusta