Application | Comment | Organism |
---|---|---|
biotechnology | development of novel formaldehyde-selective amperometric biosensors based on immobilized NAD+- and glutathione dependent formaldehyde dehydrogenase with high selectivity to formaldehyde and a low cross-sensitivity to other substances, the laboratory prototype of the sensor is applied for FA testing in some real samples of pharmaceutical (formidron), disinfectant (descoton forte) and industrial product (formalin) | Ogataea angusta |
Cloned (Comment) | Organism |
---|---|
construction of a recombinant yeast clone originated from the recipient strain of thermotolerant methylotrophic yeast Hansenula polymorpha which is a NAD+- and glutathione dependent FdDH over-producer | Ogataea angusta |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.18 | - |
formaldehyde | KM value of the used FdDH in solution from the wild strains | Ogataea angusta | |
119.9 | - |
formaldehyde | immobilized enzyme in a bioactive layer on the transducer surface, apparent Michaelis-Menten constant derived from the formaldehyde calibration curves is 120 mM with a linear detection range for formaldehyde up to 20 mM | Ogataea angusta |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
formaldehyde + NAD+ + H2O | Ogataea angusta | key enzyme of formaldehyde metabolism in microorganisms | formate + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ogataea angusta | - |
- |
- |
Purification (Comment) | Organism |
---|---|
FdDH ss isolated from the cell-free extract by a two-step ion exchange chromatography | Ogataea angusta |
Storage Stability | Organism |
---|---|
14 h and 1 mM during 22 h, the storage stability of the developed biosensors is found to be longer than 18 days at 4°C | Ogataea angusta |
the developed sensors demonstrate a good operational stability at comparatively low formaldehyde concentrations of 2 mM during | Ogataea angusta |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
formaldehyde + NAD+ + H2O | key enzyme of formaldehyde metabolism in microorganisms | Ogataea angusta | formate + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
FdDH | - |
Ogataea angusta |
formaldehyde dehydrogenase | - |
Ogataea angusta |
NAD+-dependent formaldehyde dehydrogenase | - |
Ogataea angusta |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 50 | optimal temperature for the developed FdDH-based biosensor | Ogataea angusta |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.6 | 8.3 | optimal pH-value for the developed FdDH-based biosensor | Ogataea angusta |