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Literature summary for 1.2.1.46 extracted from

  • Nikitina, O.; Shleev, S.; Gayda, G.; Demkiv, O.; Gonchar, M.; Gorton, L.; Csoeregi, E.; Nistor, M.
    Bi-enzyme biosensor based on NAD+- and glutathione-dependent recombinant formaldehyde dehydrogenase and diaphorase for formaldehyde assay (2007), Sens. Actuators B Chem., B125, 1-9.
No PubMed abstract available

Application

Application Comment Organism
industry development of a bi-enzyme biosensor for formaldehyde using diaphorase from Bacillus stearothermophilus, NAD+- and glutathione-dependent FDH as bio-recognition elements. Both enzymes can be immobilized on the top of a graphite electrode together with two osmium-redox polymers. Characteristics of the optimized formaldehyde biosensor are sensitivity, detection limit, and linear dynamic range Ogataea angusta

Organism

Organism UniProt Comment Textmining
Ogataea angusta
-
-
-
Ogataea angusta Tf 11-6
-
-
-

Purification (Commentary)

Purification (Comment) Organism
by ion-exchange chromatography Ogataea angusta

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
16.9
-
-
Ogataea angusta

Storage Stability

Storage Stability Organism
-10°C Ogataea angusta

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
formaldehyde + NAD+ + H2O
-
Ogataea angusta formate + NADH + H+
-
?
formaldehyde + NAD+ + H2O
-
Ogataea angusta Tf 11-6 formate + NADH + H+
-
?

Synonyms

Synonyms Comment Organism
FDH
-
Ogataea angusta
NAD+- and glutathione-dependent formaldehyde dehydrogenase
-
Ogataea angusta

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
maximum response of the biosensor Ogataea angusta

pH Stability

pH Stability pH Stability Maximum Comment Organism
additional information
-
FDH is not stable at a pH below 6.5, but shows higher stability in an alkaline pH domain Ogataea angusta

Cofactor

Cofactor Comment Organism Structure
glutathione
-
Ogataea angusta
NAD+
-
Ogataea angusta