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Literature summary for 1.2.1.46 extracted from

  • Demkiv, O.M.; Paryzhak, S.Y.; Gayda, G.Z.; Sibirny, V.A.; Gonchar, M.V.
    Formaldehyde dehydrogenase from the recombinant yeast Hansenula polymorpha: isolation and bioanalytic application (2007), FEMS Yeast Res., 7, 1153-1159.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
methanol cultivation on 1% methanol provides the highest level of enzyme synthesis for recipient and overproducing strains Ogataea angusta

Application

Application Comment Organism
industry high thermostability makes FdDH potentially useful for bioanalytic purposes, namely, for formaldehyde assay in food products, wastewater, and pharmaceuticals, and for biotransformation of formaldehyde to formic acid Ogataea angusta

Cloned(Commentary)

Cloned (Comment) Organism
FLD1 gene with its own promoter is recloned into LEU2-containing integrative plasmid pYT1 devoid of the autonomously replicating sequence to be used for multicopy integration of the gene into chromosome of the recipient strain NCYC 495 Ogataea angusta

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
2 * 40000, SDS-PAGE Ogataea angusta

Organism

Organism UniProt Comment Textmining
Ogataea angusta
-
stable recombinant strain Tf 11-6
-

Purification (Commentary)

Purification (Comment) Organism
by two-step anion-exchange chromatography and precipitation by 80% (NH4)2SO4, precipitate, 3.8fold purified Ogataea angusta

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.5
-
the stable recombinant strain Tf 11-6 in cell-free extract Ogataea angusta
7
-
FdDH specific activity in cell-free extract of Tf 11-6, cultivated in methanol medium supplemented with 10 mM formaldehyde Ogataea angusta
17
-
3.8fold purified enzyme at 25°C Ogataea angusta
27
-
3.8fold purified enzyme at 37°C Ogataea angusta

Storage Stability

Storage Stability Organism
-10°C, ammonium sulfate solution (80% saturation), 2 mM DTT Ogataea angusta

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
formaldehyde + NAD+ + H2O
-
Ogataea angusta formate + NADH + H+
-
?
formaldehyde + NAD+ + H2O
-
Ogataea angusta NCYC 495 formate + NADH + H+
-
?

Subunits

Subunits Comment Organism
dimer 2 * 40000, SDS-PAGE Ogataea angusta

Synonyms

Synonyms Comment Organism
FdDH
-
Ogataea angusta

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Ogataea angusta

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 65 at 65°C, the enzyme retains about 60% of its highest activity (assay time of ca. 5 min), which is equal to the level of FdDH activity at 30°C Ogataea angusta

pH Range

pH Minimum pH Maximum Comment Organism
7.5 8.5
-
Ogataea angusta

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 8.5
-
Ogataea angusta

Cofactor

Cofactor Comment Organism Structure
glutathione dependent on Ogataea angusta
NAD+ dependent on Ogataea angusta