Activating Compound | Comment | Organism | Structure |
---|---|---|---|
methanol | cultivation on 1% methanol provides the highest level of enzyme synthesis for recipient and overproducing strains | Ogataea angusta |
Application | Comment | Organism |
---|---|---|
industry | high thermostability makes FdDH potentially useful for bioanalytic purposes, namely, for formaldehyde assay in food products, wastewater, and pharmaceuticals, and for biotransformation of formaldehyde to formic acid | Ogataea angusta |
Cloned (Comment) | Organism |
---|---|
FLD1 gene with its own promoter is recloned into LEU2-containing integrative plasmid pYT1 devoid of the autonomously replicating sequence to be used for multicopy integration of the gene into chromosome of the recipient strain NCYC 495 | Ogataea angusta |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
2 * 40000, SDS-PAGE | Ogataea angusta |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ogataea angusta | - |
stable recombinant strain Tf 11-6 | - |
Purification (Comment) | Organism |
---|---|
by two-step anion-exchange chromatography and precipitation by 80% (NH4)2SO4, precipitate, 3.8fold purified | Ogataea angusta |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.5 | - |
the stable recombinant strain Tf 11-6 in cell-free extract | Ogataea angusta |
7 | - |
FdDH specific activity in cell-free extract of Tf 11-6, cultivated in methanol medium supplemented with 10 mM formaldehyde | Ogataea angusta |
17 | - |
3.8fold purified enzyme at 25°C | Ogataea angusta |
27 | - |
3.8fold purified enzyme at 37°C | Ogataea angusta |
Storage Stability | Organism |
---|---|
-10°C, ammonium sulfate solution (80% saturation), 2 mM DTT | Ogataea angusta |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
formaldehyde + NAD+ + H2O | - |
Ogataea angusta | formate + NADH + H+ | - |
? | |
formaldehyde + NAD+ + H2O | - |
Ogataea angusta NCYC 495 | formate + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 40000, SDS-PAGE | Ogataea angusta |
Synonyms | Comment | Organism |
---|---|---|
FdDH | - |
Ogataea angusta |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Ogataea angusta |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 65 | at 65°C, the enzyme retains about 60% of its highest activity (assay time of ca. 5 min), which is equal to the level of FdDH activity at 30°C | Ogataea angusta |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8.5 | - |
Ogataea angusta |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | 8.5 | - |
Ogataea angusta |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
glutathione | dependent on | Ogataea angusta | |
NAD+ | dependent on | Ogataea angusta |