Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | best sensitivity of the biosensor is observed in 2.5 mM borate buffer, pH 8.4 | Ogataea angusta |
Application | Comment | Organism |
---|---|---|
industry | construction of a formaldehyde-selective biosensor using NAD+- and glutathione-dependent recombinant formaldehyde dehydrogenase as a bio-recognition element immobilised on the surface of Si/SiO2/Si3N4 structure | Ogataea angusta |
Cloned (Comment) | Organism |
---|---|
FLD1 gene with the own promoter inserted into the integrative plasmid pYT1 containing LEU2 gene of Saccharomyces cerevisiae (as a selective marker). The constructed vector is used for multi-copy integration of the target gene into genome by transformation of the recipient cells of the strain NCYC 495 | Ogataea angusta |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | biosensor output signal decreases substantially with the increase of borate buffer concentration. Sensitivity of the biosensor in 2.5 mM Tris-HCl buffer is decreased dramatically | Ogataea angusta |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ogataea angusta | - |
- |
- |
Ogataea angusta Tf 11-6 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by ion-exchange chromatography | Ogataea angusta |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
12 | - |
at 20°C | Ogataea angusta |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
formaldehyde + NAD+ + H2O | - |
Ogataea angusta | formate + NADH + H+ | - |
? | |
formaldehyde + NAD+ + H2O | - |
Ogataea angusta Tf 11-6 | formate + NADH + H+ | - |
? | |
formaldehyde + NAD+ + H2O | - |
Ogataea angusta Tf-11-6 | formate + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
FDH | - |
Ogataea angusta |
NAD+- and glutathione-dependent formaldehyde dehydrogenase | - |
Ogataea angusta |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.4 | - |
best sensitivity of the biosensor output signal is observed in 2.5 mM borate buffer, pH 8.4 | Ogataea angusta |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
glutathione | - |
Ogataea angusta | |
NAD+ | - |
Ogataea angusta |