Cloned (Comment) | Organism |
---|---|
for expression in Escherichia coli BL21 cells | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
formaldehyde dehydrogenase-adenosine 5'-diphosphate ribose and E67L-NADH binary complexes are determined | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
E67L | mutant shows, that Glu67 is critical for capturing the substrates for catalysis | Homo sapiens |
R368L | mutant shows, that the predominant role of Arg-368 is in the binding of the coenzyme | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dodecanoic acid | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0017 | - |
S-hydroxymethylglutathione | oxidation of S-hydroxymethylglutathione, formaldehyde dehydrogenase | Homo sapiens | |
0.002 | - |
NADH | oxidation of NADH, formaldehyde dehydrogenase | Homo sapiens | |
0.0033 | - |
NAD+ | reduction of NAD+, formaldehyde dehydrogenase | Homo sapiens | |
0.0048 | - |
S-nitrosoglutathione | reduction of S-nitrosoglutathione, formaldehyde dehydrogenase | Homo sapiens | |
0.011 | - |
NADH | oxidation of NADH, R368L mutant | Homo sapiens | |
0.0144 | - |
NAD+ | reduction of NAD+, R368L mutant | Homo sapiens | |
0.038 | - |
S-hydroxymethylglutathione | oxidation of S-hydroxymethylglutathione, R368L mutant | Homo sapiens | |
0.116 | - |
NAD+ | reduction of NAD+, E67L mutant | Homo sapiens | |
0.16 | - |
S-nitrosoglutathione | reduction of S-nitrosoglutathione, R368L mutant | Homo sapiens | |
2.65 | - |
S-hydroxymethylglutathione | oxidation of S-hydroxymethylglutathione, E67L mutant | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | - |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
formaldehyde + NAD+ + H2O | Homo sapiens | - |
formate + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DE52, Q-sepharose and Sephacryl S-100HR chromatographic steps | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
formaldehyde + NAD+ + H2O | - |
Homo sapiens | formate + NADH + H+ | - |
? | |
S-hydroxymethylglutathione + NAD+ + H2O | - |
Homo sapiens | S-formylglutathione + NADH + H+ | - |
? | |
S-nitrosoglutathione + NADH + H2O | - |
Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | - |
Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
FDH | - |
Homo sapiens |
formaldehyde dehydrogenase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
S-nitrosoglutathione | Homo sapiens |
30 | - |
S-hydroxymethylglutathione | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.148 | - |
dodecanoic acid | formaldehyde dehydrogenase | Homo sapiens | |
0.161 | - |
dodecanoic acid | R368L mutant | Homo sapiens | |
0.617 | - |
dodecanoic acid | E67L mutant | Homo sapiens |