Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Pseudomonas putida |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-Methylpyrazole | native and recombinant protein | Pseudomonas putida |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zinc | structural zinc-liganding amino acids: Cys-97, Cys-100, Cys-103, Cys-111 | Pseudomonas putida | |
Zinc | active-site zinc-liganding amino acids: Cys-47 and His-68 | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
4 * 42000, recombinant protein, expressed in Escherichia coli, SDS-PAGE, calculation from gene sequence | Pseudomonas putida |
150000 | - |
recombinant protein, expressed in E. coli, gel filtration | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas putida |
recombinant protein, expressed in Escherichia coli | Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
24.1 | - |
recombinant protein, expressed in Escherichia coli | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
formaldehyde + NAD+ + H2O | - |
Pseudomonas putida | formate + NADH | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 42000, recombinant protein, expressed in Escherichia coli, SDS-PAGE, calculation from gene sequence | Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | coenzyme binding domain: Gly-Xaa-Gly-Xaa-Xaa-Gly | Pseudomonas putida |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
4-Methylpyrazole | native and recombinant protein | Pseudomonas putida |