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Literature summary for 1.2.1.46 extracted from

  • Attwood, M.M.
    Formaldehyde dehydrogenases from methylotrophs (1990), Methods Enzymol., 188, 314-330.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
2,2'-bipyridine
-
Arthrobacter sp.
8-hydroxyquinoline
-
Arthrobacter sp.
o-phenanthroline
-
Arthrobacter sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.074
-
formaldehyde
-
Arthrobacter sp.
0.45
-
NAD+
-
Arthrobacter sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43500
-
gel filtration Arthrobacter sp.
48000
-
1 * 48000, SDS-PAGE Arthrobacter sp.

Organism

Organism UniProt Comment Textmining
Arthrobacter sp.
-
P1 NCIB 11625
-
Methylophilus methylotrophus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Arthrobacter sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Arthrobacter sp.

Storage Stability

Storage Stability Organism
-20°C, stable for at least 1 month Arthrobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
formaldehyde + NAD+ + H2O
-
Arthrobacter sp. formate + NADH
-
?
formaldehyde + NAD+ + H2O
-
Methylophilus methylotrophus formate + NADH
-
?
additional information no substrates are propionaldehyde, n-butyraldehyde, isobutyraldehyde, valeraldehyde, acetaldehyde, benzaldehyde Arthrobacter sp. ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 48000, SDS-PAGE Arthrobacter sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
stable for 6 min Arthrobacter sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.1 8.5
-
Arthrobacter sp.

Cofactor

Cofactor Comment Organism Structure
additional information no activity with NADP+ Arthrobacter sp.
NAD+
-
Arthrobacter sp.