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Literature summary for 1.2.1.44 extracted from

  • Sonawane, P.; Vishwakarma, R.K.; Khan, B.M.
    Biochemical characterization of recombinant cinnamoyl CoA reductase 1 (Ll-CCRH1) from Leucaena leucocephala (2013), Int. J. Biol. Macromol., 58, 154-159.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Triton X-100 106% activity at 1% (v/v) Leucaena leucocephala
Tween 20 113% activity at 1% (v/v) Leucaena leucocephala

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Leucaena leucocephala

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 86% residual activity at 2 mM Leucaena leucocephala
Co2+ 42% residual activity at 2 mM Leucaena leucocephala
Cs2+ 83% residual activity at 2 mM Leucaena leucocephala
Cu2+ 49% residual activity at 2 mM Leucaena leucocephala
Fe2+ 92% residual activity at 2 mM Leucaena leucocephala
Hg2+ complete inhibition at 2 mM Leucaena leucocephala
Mg2+ 74% residual activity at 2 mM Leucaena leucocephala
Mn2+ 21% residual activity at 2 mM Leucaena leucocephala
Ni2+ 25% residual activity at 2 mM Leucaena leucocephala
SDS 6% residual activity at 1% (v/v) Leucaena leucocephala
Zn2+ 45% residual activity at 2 mM Leucaena leucocephala

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.031
-
coniferaldehyde in 100 mM phosphate buffer pH 7.8, at 30°C Leucaena leucocephala
0.036
-
feruloyl-CoA in 100 mM phosphate buffer pH 6.5, at 30°C Leucaena leucocephala
0.039
-
sinapaldehyde in 100 mM phosphate buffer pH 7.8, at 30°C Leucaena leucocephala
0.048
-
caffeoyl-CoA in 100 mM phosphate buffer pH 6.5, at 30°C Leucaena leucocephala
0.05
-
coniferaldehyde in 100 mM phosphate buffer pH 6.5, at 30°C Leucaena leucocephala
0.057
-
sinapoyl-CoA in 100 mM phosphate buffer pH 6.5, at 30°C Leucaena leucocephala
0.06
-
coumaroyl-CoA in 100 mM phosphate buffer pH 6.5, at 30°C Leucaena leucocephala
0.067
-
sinapaldehyde in 100 mM phosphate buffer pH 6.5, at 30°C Leucaena leucocephala

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
x * 38000, SDS-PAGE Leucaena leucocephala

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
caffeoyl-CoA + NADPH + H+ Leucaena leucocephala
-
caffealdehyde + CoA + NADP+
-
r
coniferaldehyde + CoA + NADP+ Leucaena leucocephala
-
feruloyl-CoA + NADPH + H+
-
r
coumaroyl-CoA + NADPH + H+ Leucaena leucocephala
-
coumaric aldehyde + CoA + NADP+
-
r
feruloyl-CoA + NADPH + H+ Leucaena leucocephala best substrate coniferaldehyde + CoA + NADP+
-
r
sinapaldehyde + CoA + NADP+ Leucaena leucocephala
-
sinapoyl-CoA + NADPH + H+
-
r
sinapoyl-CoA + NADPH + H+ Leucaena leucocephala
-
sinapaldehyde + CoA + NADP+
-
r

Organism

Organism UniProt Comment Textmining
Leucaena leucocephala
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Leucaena leucocephala

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
caffeoyl-CoA + NADPH + H+
-
Leucaena leucocephala caffealdehyde + CoA + NADP+
-
r
coniferaldehyde + CoA + NADP+
-
Leucaena leucocephala feruloyl-CoA + NADPH + H+
-
r
coumaroyl-CoA + NADPH + H+
-
Leucaena leucocephala coumaric aldehyde + CoA + NADP+
-
r
feruloyl-CoA + NADPH + H+ best substrate Leucaena leucocephala coniferaldehyde + CoA + NADP+
-
r
sinapaldehyde + CoA + NADP+
-
Leucaena leucocephala sinapoyl-CoA + NADPH + H+
-
r
sinapoyl-CoA + NADPH + H+
-
Leucaena leucocephala sinapaldehyde + CoA + NADP+
-
r

Subunits

Subunits Comment Organism
? x * 38000, SDS-PAGE Leucaena leucocephala

Synonyms

Synonyms Comment Organism
CCRH1
-
Leucaena leucocephala
cinnamoyl CoA reductase 1
-
Leucaena leucocephala

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
below and above 25°C, gradual loss in the enzyme activity is recorded Leucaena leucocephala

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 40 the enzyme is stable for 60 min at 25°C and at optimum temperature 30°C, stability is observed for 40 min. However, at 20°C and 35°C, 36% and 42% loss in activity is observed after 60 min. Above 40°C, significant loss in Ll-CCRH1 activity is observed within 30 min Leucaena leucocephala

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
81
-
sinapaldehyde in 100 mM phosphate buffer pH 6.5, at 30°C Leucaena leucocephala
94
-
coniferaldehyde in 100 mM phosphate buffer pH 6.5, at 30°C Leucaena leucocephala
105
-
coumaroyl-CoA in 100 mM phosphate buffer pH 6.5, at 30°C Leucaena leucocephala
113
-
caffeoyl-CoA in 100 mM phosphate buffer pH 6.5, at 30°C Leucaena leucocephala
131
-
sinapoyl-CoA in 100 mM phosphate buffer pH 6.5, at 30°C Leucaena leucocephala
164
-
feruloyl-CoA in 100 mM phosphate buffer pH 6.5, at 30°C Leucaena leucocephala
210
-
sinapaldehyde in 100 mM phosphate buffer pH 7.8, at 30°C Leucaena leucocephala
236
-
coniferaldehyde in 100 mM phosphate buffer pH 7.8, at 30°C Leucaena leucocephala

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
forward reaction Leucaena leucocephala
7.8
-
reverse reaction Leucaena leucocephala

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 7 the enzyme is most stable around pH 6.5 at 25°C for 90 min, while at pH 6.0 and 7.0, the enzyme is stable for 45 and 60 min, respectively Leucaena leucocephala

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Leucaena leucocephala
NADPH
-
Leucaena leucocephala

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1200
-
sinapaldehyde in 100 mM phosphate buffer pH 6.5, at 30°C Leucaena leucocephala
1700
-
coumaroyl-CoA in 100 mM phosphate buffer pH 6.5, at 30°C Leucaena leucocephala
1900
-
coniferaldehyde in 100 mM phosphate buffer pH 6.5, at 30°C Leucaena leucocephala
2300
-
sinapoyl-CoA in 100 mM phosphate buffer pH 6.5, at 30°C Leucaena leucocephala
2400
-
caffeoyl-CoA in 100 mM phosphate buffer pH 6.5, at 30°C Leucaena leucocephala
4600
-
feruloyl-CoA in 100 mM phosphate buffer pH 6.5, at 30°C Leucaena leucocephala
5300
-
sinapaldehyde in 100 mM phosphate buffer pH 7.8, at 30°C Leucaena leucocephala
7500
-
coniferaldehyde in 100 mM phosphate buffer pH 7.8, at 30°C Leucaena leucocephala