Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Triton X-100 | 106% activity at 1% (v/v) | Leucaena leucocephala | |
Tween 20 | 113% activity at 1% (v/v) | Leucaena leucocephala |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Leucaena leucocephala |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 86% residual activity at 2 mM | Leucaena leucocephala | |
Co2+ | 42% residual activity at 2 mM | Leucaena leucocephala | |
Cs2+ | 83% residual activity at 2 mM | Leucaena leucocephala | |
Cu2+ | 49% residual activity at 2 mM | Leucaena leucocephala | |
Fe2+ | 92% residual activity at 2 mM | Leucaena leucocephala | |
Hg2+ | complete inhibition at 2 mM | Leucaena leucocephala | |
Mg2+ | 74% residual activity at 2 mM | Leucaena leucocephala | |
Mn2+ | 21% residual activity at 2 mM | Leucaena leucocephala | |
Ni2+ | 25% residual activity at 2 mM | Leucaena leucocephala | |
SDS | 6% residual activity at 1% (v/v) | Leucaena leucocephala | |
Zn2+ | 45% residual activity at 2 mM | Leucaena leucocephala |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.031 | - |
coniferaldehyde | in 100 mM phosphate buffer pH 7.8, at 30°C | Leucaena leucocephala | |
0.036 | - |
feruloyl-CoA | in 100 mM phosphate buffer pH 6.5, at 30°C | Leucaena leucocephala | |
0.039 | - |
sinapaldehyde | in 100 mM phosphate buffer pH 7.8, at 30°C | Leucaena leucocephala | |
0.048 | - |
caffeoyl-CoA | in 100 mM phosphate buffer pH 6.5, at 30°C | Leucaena leucocephala | |
0.05 | - |
coniferaldehyde | in 100 mM phosphate buffer pH 6.5, at 30°C | Leucaena leucocephala | |
0.057 | - |
sinapoyl-CoA | in 100 mM phosphate buffer pH 6.5, at 30°C | Leucaena leucocephala | |
0.06 | - |
coumaroyl-CoA | in 100 mM phosphate buffer pH 6.5, at 30°C | Leucaena leucocephala | |
0.067 | - |
sinapaldehyde | in 100 mM phosphate buffer pH 6.5, at 30°C | Leucaena leucocephala |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
x * 38000, SDS-PAGE | Leucaena leucocephala |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
caffeoyl-CoA + NADPH + H+ | Leucaena leucocephala | - |
caffealdehyde + CoA + NADP+ | - |
r | |
coniferaldehyde + CoA + NADP+ | Leucaena leucocephala | - |
feruloyl-CoA + NADPH + H+ | - |
r | |
coumaroyl-CoA + NADPH + H+ | Leucaena leucocephala | - |
coumaric aldehyde + CoA + NADP+ | - |
r | |
feruloyl-CoA + NADPH + H+ | Leucaena leucocephala | best substrate | coniferaldehyde + CoA + NADP+ | - |
r | |
sinapaldehyde + CoA + NADP+ | Leucaena leucocephala | - |
sinapoyl-CoA + NADPH + H+ | - |
r | |
sinapoyl-CoA + NADPH + H+ | Leucaena leucocephala | - |
sinapaldehyde + CoA + NADP+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leucaena leucocephala | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Leucaena leucocephala |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
caffeoyl-CoA + NADPH + H+ | - |
Leucaena leucocephala | caffealdehyde + CoA + NADP+ | - |
r | |
coniferaldehyde + CoA + NADP+ | - |
Leucaena leucocephala | feruloyl-CoA + NADPH + H+ | - |
r | |
coumaroyl-CoA + NADPH + H+ | - |
Leucaena leucocephala | coumaric aldehyde + CoA + NADP+ | - |
r | |
feruloyl-CoA + NADPH + H+ | best substrate | Leucaena leucocephala | coniferaldehyde + CoA + NADP+ | - |
r | |
sinapaldehyde + CoA + NADP+ | - |
Leucaena leucocephala | sinapoyl-CoA + NADPH + H+ | - |
r | |
sinapoyl-CoA + NADPH + H+ | - |
Leucaena leucocephala | sinapaldehyde + CoA + NADP+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 38000, SDS-PAGE | Leucaena leucocephala |
Synonyms | Comment | Organism |
---|---|---|
CCRH1 | - |
Leucaena leucocephala |
cinnamoyl CoA reductase 1 | - |
Leucaena leucocephala |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
below and above 25°C, gradual loss in the enzyme activity is recorded | Leucaena leucocephala |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 40 | the enzyme is stable for 60 min at 25°C and at optimum temperature 30°C, stability is observed for 40 min. However, at 20°C and 35°C, 36% and 42% loss in activity is observed after 60 min. Above 40°C, significant loss in Ll-CCRH1 activity is observed within 30 min | Leucaena leucocephala |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
81 | - |
sinapaldehyde | in 100 mM phosphate buffer pH 6.5, at 30°C | Leucaena leucocephala | |
94 | - |
coniferaldehyde | in 100 mM phosphate buffer pH 6.5, at 30°C | Leucaena leucocephala | |
105 | - |
coumaroyl-CoA | in 100 mM phosphate buffer pH 6.5, at 30°C | Leucaena leucocephala | |
113 | - |
caffeoyl-CoA | in 100 mM phosphate buffer pH 6.5, at 30°C | Leucaena leucocephala | |
131 | - |
sinapoyl-CoA | in 100 mM phosphate buffer pH 6.5, at 30°C | Leucaena leucocephala | |
164 | - |
feruloyl-CoA | in 100 mM phosphate buffer pH 6.5, at 30°C | Leucaena leucocephala | |
210 | - |
sinapaldehyde | in 100 mM phosphate buffer pH 7.8, at 30°C | Leucaena leucocephala | |
236 | - |
coniferaldehyde | in 100 mM phosphate buffer pH 7.8, at 30°C | Leucaena leucocephala |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
forward reaction | Leucaena leucocephala |
7.8 | - |
reverse reaction | Leucaena leucocephala |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 7 | the enzyme is most stable around pH 6.5 at 25°C for 90 min, while at pH 6.0 and 7.0, the enzyme is stable for 45 and 60 min, respectively | Leucaena leucocephala |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Leucaena leucocephala | |
NADPH | - |
Leucaena leucocephala |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1200 | - |
sinapaldehyde | in 100 mM phosphate buffer pH 6.5, at 30°C | Leucaena leucocephala | |
1700 | - |
coumaroyl-CoA | in 100 mM phosphate buffer pH 6.5, at 30°C | Leucaena leucocephala | |
1900 | - |
coniferaldehyde | in 100 mM phosphate buffer pH 6.5, at 30°C | Leucaena leucocephala | |
2300 | - |
sinapoyl-CoA | in 100 mM phosphate buffer pH 6.5, at 30°C | Leucaena leucocephala | |
2400 | - |
caffeoyl-CoA | in 100 mM phosphate buffer pH 6.5, at 30°C | Leucaena leucocephala | |
4600 | - |
feruloyl-CoA | in 100 mM phosphate buffer pH 6.5, at 30°C | Leucaena leucocephala | |
5300 | - |
sinapaldehyde | in 100 mM phosphate buffer pH 7.8, at 30°C | Leucaena leucocephala | |
7500 | - |
coniferaldehyde | in 100 mM phosphate buffer pH 7.8, at 30°C | Leucaena leucocephala |