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Literature summary for 1.2.1.44 extracted from

  • Baltas, M.; Lapeyre, C.; Bedos-Belval, F.; Maturano, M.; Saint-Aguet, P.; Roussel, L.; Duran, H.; Grima-Pettenati, J.
    Kinetic and inhibition studies of cinnamoyl-CoA reductase 1 from Arabidopsis thaliana (2005), Plant Physiol. Biochem., 43, 746-753.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
into the pGEX-4T vector for expression in Escherichia coli BL21DE3 cells Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
S-[2-(acetylamino)ethyl] O-ethyl [(E)-2-(3,4-dimethoxyphenyl)ethenyl]phosphonothioate
-
Arabidopsis thaliana
S-[2-(acetylamino)ethyl] O-ethyl [(E)-2-(4-hydroxy-3,5-dimethoxyphenyl)ethenyl]phosphonothioate
-
Arabidopsis thaliana
S-[2-(acetylamino)ethyl] O-ethyl [(E)-2-(4-hydroxy-3-methoxyphenyl)ethenyl]phosphonothioate
-
Arabidopsis thaliana
S-[2-(acetylamino)ethyl] O-ethyl [(E)-2-(4-hydroxyphenyl)ethenyl]phosphonothioate
-
Arabidopsis thaliana
S-[2-(acetylamino)ethyl] O-ethyl [(E)-2-(4-methoxyphenyl)ethenyl]phosphonothioate
-
Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00042
-
feruloyl-CoA
-
Arabidopsis thaliana
0.00055
-
sinapoyl-CoA
-
Arabidopsis thaliana
0.00227
-
p-Coumaroyl-CoA
-
Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
determined by SDS-PAGE Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cinnamaldehyde + CoA + NADP+ Arabidopsis thaliana
-
cinnamoyl-CoA + NADPH + H+
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using a glutathione-sepharose 4 Fast Flow column, purified GST-AtCCR1 is cleaved by thrombin to remove the GST tag Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cinnamaldehyde + CoA + NADP+
-
Arabidopsis thaliana cinnamoyl-CoA + NADPH + H+
-
?
feruloyl-CoA + NADPH + H+
-
Arabidopsis thaliana coniferaldehyde + CoA + NADP+
-
?
p-coumaroyl-CoA + NADPH + H+
-
Arabidopsis thaliana p-coumaraldehyde + CoA + NADP+
-
?
sinapoyl-CoA + NADPH + H+
-
Arabidopsis thaliana sinapaldehyde + CoA + NADP+
-
?

Synonyms

Synonyms Comment Organism
AtCCR1
-
Arabidopsis thaliana
CCR ambiguous Arabidopsis thaliana
cinnamoyl-CoA reductase
-
Arabidopsis thaliana
cinnamoyl-CoA reductase 1
-
Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
activity assay Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.63
-
p-Coumaroyl-CoA
-
Arabidopsis thaliana
1.67
-
sinapoyl-CoA
-
Arabidopsis thaliana
1.9
-
feruloyl-CoA
-
Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.25
-
activity assay Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Arabidopsis thaliana

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0044
-
S-[2-(acetylamino)ethyl] O-ethyl [(E)-2-(4-hydroxy-3-methoxyphenyl)ethenyl]phosphonothioate with feruloyl-CoA as substrate, noncompetitive inhibition Arabidopsis thaliana
0.0071
-
S-[2-(acetylamino)ethyl] O-ethyl [(E)-2-(4-hydroxy-3,5-dimethoxyphenyl)ethenyl]phosphonothioate with sinapoyl-CoA as substrate, noncompetitive inhibition Arabidopsis thaliana
0.031
-
S-[2-(acetylamino)ethyl] O-ethyl [(E)-2-(3,4-dimethoxyphenyl)ethenyl]phosphonothioate with feruloyl-CoA as substrate, uncompetitive inhibition Arabidopsis thaliana
0.055
-
S-[2-(acetylamino)ethyl] O-ethyl [(E)-2-(4-hydroxy-3,5-dimethoxyphenyl)ethenyl]phosphonothioate with feruloyl-CoA as substrate, uncompetitive inhibition Arabidopsis thaliana
0.057
-
S-[2-(acetylamino)ethyl] O-ethyl [(E)-2-(4-hydroxy-3-methoxyphenyl)ethenyl]phosphonothioate with sinapoyl-CoA as substrate, noncompetitive inhibition Arabidopsis thaliana
0.068
-
S-[2-(acetylamino)ethyl] O-ethyl [(E)-2-(4-methoxyphenyl)ethenyl]phosphonothioate with sinapoyl-CoA as substrate, noncompetitive inhibition Arabidopsis thaliana
0.156
-
S-[2-(acetylamino)ethyl] O-ethyl [(E)-2-(3,4-dimethoxyphenyl)ethenyl]phosphonothioate with sinapoyl-CoA as substrate, uncompetitive inhibition Arabidopsis thaliana
0.212
-
S-[2-(acetylamino)ethyl] O-ethyl [(E)-2-(4-hydroxyphenyl)ethenyl]phosphonothioate with sinapoyl-CoA as substrate, uncompetitive inhibition Arabidopsis thaliana
0.29
-
S-[2-(acetylamino)ethyl] O-ethyl [(E)-2-(4-methoxyphenyl)ethenyl]phosphonothioate with feruloyl-CoA as substrate, uncompetitive inhibition Arabidopsis thaliana
0.422
-
S-[2-(acetylamino)ethyl] O-ethyl [(E)-2-(4-hydroxyphenyl)ethenyl]phosphonothioate with feruloyl-CoA as substrate, uncompetitive inhibition Arabidopsis thaliana