Application | Comment | Organism |
---|---|---|
degradation | ALDH1A1 appears to be the major if not the only enzyme responsible for the oxidation of 3-deoxyglucosone to 2-keto-3-deoxygluconate | Mus musculus |
degradation | urinary excretion of 2-keto-3-deoxygluconate amounts to 16.7 micromol/g creatinine in humans, indicating that 3-deoxyglucosone may be quantitatively a more important substrate than retinaldehyde for ALDH1A1 | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
ligated into a pCMV5 expression vector and overexpressed in HEK-293 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | 3-deoxyglucosone dehydrogenase activity is undetectable in tissues from ALDH1A1-/- mice | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
benzaldehyde | - |
Homo sapiens | |
0.085 | - |
NAD+ | partially purified recombinant ALDH1A1 at pH 7.1 and in the presence of 0.1 mM 3-deoxyglucosone | Homo sapiens | |
0.095 | - |
3-deoxyglucosone | partially purified recombinant ALDH1A1 at pH 7.1 and in the presence of 1 mM NAD | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
sequence analysis | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Mus musculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by gel filtration, more than 400fold with a yield of about 1%, to near homogeneity | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
erythrocyte | - |
Mus musculus | - |
erythrocyte | - |
Homo sapiens | - |
liver | highest activity | Mus musculus | - |
lung | intermediate activity | Mus musculus | - |
additional information | no activity is detectable in brain, thymus, spleen, kidneys, skeletal muscle and heart | Mus musculus | - |
testis | intermediate activity | Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-deoxyglucosone + NAD+ + H2O | - |
Mus musculus | 2-keto-3-deoxygluconate + NADH + H+ | - |
? | |
3-deoxyglucosone + NAD+ + H2O | - |
Homo sapiens | 2-keto-3-deoxygluconate + NADH + H+ | - |
? | |
benzaldehyde + NAD+ + H2O | - |
Homo sapiens | benzoate + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
aldehyde dehydrogenase 1A1 | - |
Mus musculus |
aldehyde dehydrogenase 1A1 | - |
Homo sapiens |
ALDH1A1 | - |
Mus musculus |
ALDH1A1 | - |
Homo sapiens |
retinaldehyde dehydrogenase 1 | - |
Mus musculus |
retinaldehyde dehydrogenase 1 | - |
Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.1 | 9 | - |
Homo sapiens |