Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | stimulation of the reduction of the intermediate benzoyladenosine 5'-monophosphate to benzaldehyde | Neurospora crassa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AMP | inhibition of the exchange reaction of diphosphate and ATP | Neurospora crassa | |
hydroxylamine | inhibition of the exchange reaction of diphosphate and ATP | Neurospora crassa | |
iodoacetamide | inhibition of the reduction of benzoyladenosine 5'-monophosphate, even in the presence of dithiothreitol | Neurospora crassa | |
iodoacetate | inhibition of the reduction of benzoyladenosine 5'-monophosphate, even in the presence of dithiothreitol | Neurospora crassa | |
N-ethylmaleimide | inhibition of the reduction of benzoyladenosine 5'-monophosphate | Neurospora crassa | |
NADPH | inhibition of the exchange reaction of diphosphate and ATP, inhibitory effect increases when dithiothreitol is added | Neurospora crassa | |
o-Iodosobenzoate | inhibition of the reduction of benzoyladenosine 5'-monophosphate, even in the presence of dithiothreitol | Neurospora crassa | |
p-chloromercuribenzoate | inhibition of the reduction of benzoyladenosine 5'-monophosphate, even in the presence of dithiothreitol | Neurospora crassa | |
p-diazobenzenesulfonate | inhibition of the reduction of benzoyladenosine 5'-monophosphate, even in the presence of dithiothreitol | Neurospora crassa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.063 | - |
benzoate | - |
Neurospora crassa | |
0.13 | - |
NADPH | reduction of the intermediate benzoyladenylate to benzaldehyde | Neurospora crassa | |
0.2 | - |
benzoyladenosine 5'-monophosphate | - |
Neurospora crassa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Neurospora crassa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
aromatic acids + NADPH + H+ + ATP | Neurospora crassa | - |
aromatic aldehydes + NADP+ + AMP + diphosphate + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Neurospora crassa | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Neurospora crassa | - |
Storage Stability | Organism |
---|---|
pH 7.6, 50% glycerol, 1 mM EDTA, 2.5 mM mercaptoethanol, 6 months | Neurospora crassa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
aromatic acids + NADPH + H+ + ATP | - |
Neurospora crassa | aromatic aldehydes + NADP+ + AMP + diphosphate + H2O | - |
? | |
aromatic acids + NADPH + H+ + ATP | - |
Neurospora crassa | aromatic aldehydes + NADP+ + AMP + diphosphate + H2O | - |
r | |
benzaldehyde + NADP+ + benzoyladenosine 5'-monophosphate + phosphate | - |
Neurospora crassa | benzoate + NADPH + ATP | - |
r | |
benzoic acid + NADPH + H+ + ATP | the reaction proceeds via benzoyladenosine 5'-monophosphate which is further reduced by NADPH to benzaldehyde | Neurospora crassa | benzaldehyde + NADP+ + AMP + diphosphate | - |
? | |
cinnamic acid + NADPH + ATP | - |
Neurospora crassa | 3-phenyl-2-propen-1-al + NADP+ + AMP + H2O | - |
? | |
m-hydroxybenzoic acid + NADPH + ATP | - |
Neurospora crassa | m-hydroxybenzaldehyde + NADP+ + AMP + H2O | - |
? | |
p-anisic acid + NADPH + ATP | - |
Neurospora crassa | p-methoxybenzaldehyde + NADP+ + AMP + H2O | - |
? | |
p-coumaric acid + NADPH + ATP | - |
Neurospora crassa | 3-(4-hydroxphenyl)-2-propen-1-al + NADP+ + AMP + H2O | - |
? | |
p-hydroxybenzoic acid + NADPH + ATP | - |
Neurospora crassa | p-hydroxybenzaldehyde + NADP+ + AMP + H2O | - |
? | |
salicylic acid + NADPH + ATP | rate relative to benzoate 9% | Neurospora crassa | salicyl aldehyde + NADP+ + AMP + H2O | - |
? | |
vanillic acid + NADPH + H+ + ATP | - |
Neurospora crassa | 4-hydroxy-3-methoxybenzaldehyde + NADP+ + AMP + diphosphate | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
for the reduction of benzoyladenosine 5'-monophosphate | Neurospora crassa |
8.4 | 9.2 | for the exchange reaction of diphosphate and ATP | Neurospora crassa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | - |
not active below, benzoate + ATP | Neurospora crassa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Neurospora crassa | |
NADPH | - |
Neurospora crassa |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
2.2 | - |
AMP | - |
Neurospora crassa |