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Literature summary for 1.2.1.28 extracted from

  • Livingstone, A.; Fewson, C.A.; Kennedy, S.I.T.; Zatman, L.J.
    Two benzaldehyde dehydrogenases in bacterium N.C.I.B. 8250. Distinguishing properties and regulation (1972), Biochem. J., 130, 927-935.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.2.1.28

Inhibitors

Inhibitors Comment Organism Structure
4-chloromercuribenzoate 0.5 mM, complete inhibition Acinetobacter calcoaceticus
N-ethylmaleimide 0.5 mM, complete inhibition Acinetobacter calcoaceticus

Metals/Ions

Metals/Ions Comment Organism Structure
Cs+ benzaldehyde dehydrogenase I Acinetobacter calcoaceticus
K+ or NH4+ or Rb+ required for maximal activity, maximum activity occurs at approx. 70 mM K+ Acinetobacter calcoaceticus
K+ only dehydrogenase I of strain N.C.I.B. 8250 is activated by univalent cations Acinetobacter calcoaceticus
Li+ benzaldehyde dehydrogenase I Acinetobacter calcoaceticus
Na+ benzaldehyde dehydrogenase I Acinetobacter calcoaceticus
NH4+ benzaldehyde dehydrogenase I Acinetobacter calcoaceticus
Rb+ benzaldehyde dehydrogenase I Acinetobacter calcoaceticus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
180000
-
 strain N.C.I.B. 8250, gel filtration Acinetobacter calcoaceticus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
benzaldehyde + NAD+ + H2O Acinetobacter calcoaceticus
-
 benzoate + NADH + H+
-
 ?
benzaldehyde + NAD+ + H2O Acinetobacter calcoaceticus N.C.I.B. 8250
-
 benzoate + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Acinetobacter calcoaceticus
-
 N.C.I.B. 8250
-
Acinetobacter calcoaceticus N.C.I.B. 8250
-
 N.C.I.B. 8250
-

Purification (Commentary)

Purification (Comment) Organism
dehydrogenases I and II Acinetobacter calcoaceticus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.002
-
 benzaldehyde dehydrogenase II and class 1 mutant activity in cell extracts of wild-type grown without inducer Acinetobacter calcoaceticus
0.006
-
 class 1 mutant enzyme activity in cell extracts of wild-type grown in the presence of 5 mM benzyl alcohol or 1 mM benzaldehyde Acinetobacter calcoaceticus
0.094
-
 class 2 mutant enzyme activity in cell extracts of wild-type grown in the presence of 1 mM benzaldehyde Acinetobacter calcoaceticus
0.126
-
 benzaldehyde dehydrogenase II activity in cell extracts of wild-type grown in the presence of 1 mM benzaldehyde Acinetobacter calcoaceticus
0.324
-
 benzaldehyde dehydrogenase II activity in cell extracts of wild-type grown in the presence of 5 mM benzyl alcohol Acinetobacter calcoaceticus
0.325
-
 class 2 mutant enzyme activity in cell extracts of wild-type grown in the presence of 5 mM benzyl alcohol Acinetobacter calcoaceticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzaldehyde + NAD+ + H2O
-
 Acinetobacter calcoaceticus benzoate + NADH + H+
-
 ?
benzaldehyde + NAD+ + H2O
-
 Acinetobacter calcoaceticus N.C.I.B. 8250 benzoate + NADH + H+
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 half-lives: benzaldehyde dehydrogenase I, 520 min, benzaldehyde dehydrogenase II, 30 min, pH 9.18, half-life for benzaldehyde dehydrogenase II increases to 113 min at pH 8.5 Acinetobacter calcoaceticus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Acinetobacter calcoaceticus