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Literature summary for 1.2.1.27 extracted from

  • Esser, D.; Kouril, T.; Talfournier, F.; Polkowska, J.; Schrader, T.; Bräsen, C.; Siebers, B.
    Unraveling the function of paralogs of the aldehyde dehydrogenase super family from Sulfolobus solfataricus (2013), Extremophiles, 17, 205-216.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Saccharolobus solfataricus
SSO1218 expressed in Escherichia coli BL21 (DE3) RIL via isopropyl-b-D-thiogalactopyranoside induction Saccharolobus solfataricus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
SDS-PAGE Saccharolobus solfataricus
54600
-
calculated from sequence Saccharolobus solfataricus
130000
-
native molecular weight using gel filtration Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-methyl-3-oxopropanoate + CoA + H2O + NAD+ Saccharolobus solfataricus
-
propanoyl-CoA + HCO3- + NADH
-
?
2-methyl-3-oxopropanoate + CoA + H2O + NAD+ Saccharolobus solfataricus P2
-
propanoyl-CoA + HCO3- + NADH
-
?

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus Q97YT9
-
-
Saccharolobus solfataricus P2 Q97YT9
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Saccharolobus solfataricus
to apparent homogeneity using heat precipitation at 80°C for 20 min, ion exchange chromatography and gel filtration Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-methyl-3-oxopropanoate + CoA + H2O + NAD+
-
Saccharolobus solfataricus propanoyl-CoA + HCO3- + NADH
-
?
2-methyl-3-oxopropanoate + CoA + H2O + NAD+
-
Saccharolobus solfataricus P2 propanoyl-CoA + HCO3- + NADH
-
?
malonate semialdehyde + CoA + H2O + NAD+
-
Saccharolobus solfataricus acetyl-CoA + CO2 + NADH
-
?
malonate semialdehyde + CoA + H2O + NAD+
-
Saccharolobus solfataricus P2 acetyl-CoA + CO2 + NADH
-
?
malonate semialdehyde + CoA + NAD+
-
Saccharolobus solfataricus acetyl-CoA + CO2 + NADH
-
?
malonate semialdehyde + CoA + NAD+
-
Saccharolobus solfataricus P2 acetyl-CoA + CO2 + NADH
-
?
methylmalonate semialdehyde + CoA + H2O + NAD+
-
Saccharolobus solfataricus propanoyl-CoA + HCO3- + NADH
-
?
additional information no activity is observed for the substrates D,L-glyceraldehyde, D,L-glyceraldehyde 3-phosphate and D,L-glycolaldehyde neither with NAD+ nor with NADP+ as cosubstrate Saccharolobus solfataricus ?
-
?
additional information no activity detected in the presence of NADP+ as cosubstrate, no activity with D,L-glyceraldehyde, D,L-glyceraldehyde-3-phosphate and D,L-glycolaldehyde - neither with NAD+ nor with NADP+ as cosubstrate Saccharolobus solfataricus ?
-
?
additional information no activity is observed for the substrates D,L-glyceraldehyde, D,L-glyceraldehyde 3-phosphate and D,L-glycolaldehyde neither with NAD+ nor with NADP+ as cosubstrate Saccharolobus solfataricus P2 ?
-
?
additional information no activity detected in the presence of NADP+ as cosubstrate, no activity with D,L-glyceraldehyde, D,L-glyceraldehyde-3-phosphate and D,L-glycolaldehyde - neither with NAD+ nor with NADP+ as cosubstrate Saccharolobus solfataricus P2 ?
-
?
propionaldehyde + CoA + H2O + NAD+ no physiological substrate Saccharolobus solfataricus ?
-
?
propionaldehyde + CoA + NAD+ highest activity, no physiological substrate for MSDH, it only forms the same thiopropionyl enzyme intermediate as methylmalonate semialdehyde and malonate semialdehyde Saccharolobus solfataricus ? + NADH
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 50000 Saccharolobus solfataricus

Synonyms

Synonyms Comment Organism
methylmalonate semialdehyde dehydrogenase
-
Saccharolobus solfataricus
MSDH
-
Saccharolobus solfataricus
SSO1218
-
Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.003
-
methylmalonate semialdehyde 30°C, pH 8.2 Saccharolobus solfataricus
0.003
-
2-Methyl-3-oxopropanoate pH 8.2, 30°C Saccharolobus solfataricus
0.02
-
malonate semialdehyde 30°C, pH 8.2 Saccharolobus solfataricus
0.02
-
malonate semialdehyde pH 8.2, 30°C Saccharolobus solfataricus
0.03
-
propionaldehyde 30°C, pH 8.2 Saccharolobus solfataricus
0.03
-
propionaldehyde pH 8.2, 30°C Saccharolobus solfataricus

Cofactor

Cofactor Comment Organism Structure
CoA enzyme is strictly CoA-dependent Saccharolobus solfataricus
NAD+ no activity in the presence of NADP+ as cosubstrate Saccharolobus solfataricus
NAD+ cosubstrate Saccharolobus solfataricus

General Information

General Information Comment Organism
metabolism for MSDH, a major function in the degradation of branched chain amino acids is proposed which is supported by the high sequence homology with characterized MSDHs from bacteria Saccharolobus solfataricus