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Literature summary for 1.2.1.27 extracted from
- Purification of methylmalonate-semialdehyde dehydrogenase from Pseudomonas aeruginosa PAO (1988), Methods Enzymol., 166, 389-393.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.021 | - |
CoA | - |
Pseudomonas aeruginosa |  |
| 0.024 | - |
2-Methyl-3-oxopropanoate | - |
Pseudomonas aeruginosa | |
| 6.4 | - |
propanal | - |
Pseudomonas aeruginosa | |
| 44 | - |
2-mercaptoethanol | - |
Pseudomonas aeruginosa | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-methyl-3-oxopropanoate + CoA + NAD+ | Pseudomonas aeruginosa | - |
propanoyl-CoA + CO2 + NADH | - |
ir | |
| 2-methyl-3-oxopropanoate + CoA + NAD+ | Pseudomonas aeruginosa PAO | - |
propanoyl-CoA + CO2 + NADH | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
| Pseudomonas aeruginosa | - |
PAO | - |
| Pseudomonas aeruginosa PAO | - |
PAO | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-methyl-3-oxopropanoate + CoA + NAD+ | - |
Pseudomonas aeruginosa | propanoyl-CoA + CO2 + NADH | - |
ir | |
| 2-methyl-3-oxopropanoate + CoA + NAD+ | other acyl acceptor: 2-mercaptoethanol | Pseudomonas aeruginosa | propanoyl-CoA + CO2 + NADH | - |
ir | |
| 2-methyl-3-oxopropanoate + CoA + NAD+ | - |
Pseudomonas aeruginosa PAO | propanoyl-CoA + CO2 + NADH | - |
ir | |
| 2-methyl-3-oxopropanoate + CoA + NAD+ | other acyl acceptor: 2-mercaptoethanol | Pseudomonas aeruginosa PAO | propanoyl-CoA + CO2 + NADH | - |
ir | |
| propanal + 2-mercaptoethanol + NAD+ | - |
Pseudomonas aeruginosa | ? + NADH | - |
ir | |
| propanal + 2-mercaptoethanol + NAD+ | - |
Pseudomonas aeruginosa PAO | ? + NADH | - |
ir | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| CoA | - |
Homo sapiens | |
| CoA | - |
Pseudomonas aeruginosa | |
| NAD+ | - |
Homo sapiens | |
| NAD+ | - |
Pseudomonas aeruginosa | |
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