Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Saccharolobus solfataricus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-oxoglutaric semialdehyde | substrate inhibition | Saccharolobus solfataricus | |
NAD+ | substrate inhibition | Saccharolobus solfataricus | |
succinate semialdehyde | substrate inhibition | Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
succinate semialdehyde | pH 6.5, 70°C, cofactor NAD+ | Saccharolobus solfataricus | |
0.3 | - |
2,5-dioxopentanoate | pH 6.5, 70°C, cofactor NAD+ | Saccharolobus solfataricus | |
9.1 | - |
NAD+ | pH 6.5, 70°C | Saccharolobus solfataricus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
3 * 50000, SDS-PAGE | Saccharolobus solfataricus |
51500 | - |
3 * 51500, calculated from sequence | Saccharolobus solfataricus |
157000 | - |
gel filtration | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | Q97XS9 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,5-dioxopentanoate + NAD+ + H2O | no activity in presence of NADP+ | Saccharolobus solfataricus | ? | - |
? | |
additional information | formaldehyde, betaine aldehyde and benzaldehyde can not serve as substrates | Saccharolobus solfataricus | ? | - |
? | |
succinate semialdehyde + NAD+ + H2O | no activity in presence of NADP+ | Saccharolobus solfataricus | succinate + NADH + 2 H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotrimer | 3 * 50000, SDS-PAGE | Saccharolobus solfataricus |
homotrimer | 3 * 51500, calculated from sequence | Saccharolobus solfataricus |
Synonyms | Comment | Organism |
---|---|---|
SSADH-I | - |
Saccharolobus solfataricus |
SSO1629 | ordered locus name | Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
assay at | Saccharolobus solfataricus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7.1 | - |
2,5-dioxopentanoate | pH 6.5, 70°C, cofactor NAD+ | Saccharolobus solfataricus | |
22.6 | - |
succinate semialdehyde | pH 6.5, 70°C, cofactor NAD+ | Saccharolobus solfataricus | |
69 | - |
NAD+ | pH 6.5, 70°C | Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
assay at | Saccharolobus solfataricus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | no activity in presence of NADP+ | Saccharolobus solfataricus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7.6 | - |
NAD+ | pH 6.5, 70°C | Saccharolobus solfataricus | |
21.4 | - |
2,5-dioxopentanoate | pH 6.5, 70°C, cofactor NAD+ | Saccharolobus solfataricus | |
842 | - |
succinate semialdehyde | pH 6.5, 70°C, cofactor NAD+ | Saccharolobus solfataricus |