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Literature summary for 1.2.1.20 extracted from

  • Chang, Y.F.; Adams, E.
    Factors influencing growth on L-lysine by Pseudomonas (1971), Methods Enzymol., 17B, 166-171.
No PubMed abstract available
Search for more literature for 1.2.1.20

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
170000
-
 sucrose density gradient sedimentation, gel filtration Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glutarate semialdehyde + NAD+ + H2O Pseudomonas putida constitutive levels of the enzyme are produced under all growth conditions. Growth on Lys leads to specific activity levels 2-3fold higher than growth on other amino acids glutarate + NADH
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas putida

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.5
-
-
 Pseudomonas putida

Storage Stability

Storage Stability Organism
0°C, unstable to storage, inclusion of 10% glycerol greatly improves stability Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-hydroxybutyraldehyde + NAD+ + H2O low activity Pseudomonas putida 2-hydroxybutanoate + NADH
-
 ?
2-oxoglutaric semialdehyde + NAD+ + H2O
-
 Pseudomonas putida 2-oxoglutarate + NADH
-
 ?
5-hydroxyvaleraldehyde + NAD+ + H2O low activity Pseudomonas putida 5-hydroxypentanoate + NADH + H+
-
 ?
butyraldehyde + NAD+ + H2O low activity Pseudomonas putida butanoate + NADH
-
 ?
DL-glyceraldehyde + NAD+ + H2O low activity Pseudomonas putida glycerate + NADH
-
 ?
glutaraldehyde + NAD+ + H2O low activity Pseudomonas putida glutarate semialdehyde + NADH
-
 ?
glutarate semialdehyde + NAD+ + H2O
-
 Pseudomonas putida glutarate + NADH
-
 ?
glutarate semialdehyde + NAD+ + H2O constitutive levels of the enzyme are produced under all growth conditions. Growth on Lys leads to specific activity levels 2-3fold higher than growth on other amino acids Pseudomonas putida glutarate + NADH
-
 ?
heptaldehyde + NAD+ + H2O low activity Pseudomonas putida heptanoate + NADH
-
 ?
valeraldehyde + NAD+ + H2O low activity Pseudomonas putida valeric acid + NADH + H+
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
NAD+ coenzyme Pseudomonas putida