Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
170000 | - |
sucrose density gradient sedimentation, gel filtration | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutarate semialdehyde + NAD+ + H2O | Pseudomonas putida | constitutive levels of the enzyme are produced under all growth conditions. Growth on Lys leads to specific activity levels 2-3fold higher than growth on other amino acids | glutarate + NADH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Pseudomonas putida |
Storage Stability | Organism |
---|---|
0°C, unstable to storage, inclusion of 10% glycerol greatly improves stability | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-hydroxybutyraldehyde + NAD+ + H2O | low activity | Pseudomonas putida | 2-hydroxybutanoate + NADH | - |
? | |
2-oxoglutaric semialdehyde + NAD+ + H2O | - |
Pseudomonas putida | 2-oxoglutarate + NADH | - |
? | |
5-hydroxyvaleraldehyde + NAD+ + H2O | low activity | Pseudomonas putida | 5-hydroxypentanoate + NADH + H+ | - |
? | |
butyraldehyde + NAD+ + H2O | low activity | Pseudomonas putida | butanoate + NADH | - |
? | |
DL-glyceraldehyde + NAD+ + H2O | low activity | Pseudomonas putida | glycerate + NADH | - |
? | |
glutaraldehyde + NAD+ + H2O | low activity | Pseudomonas putida | glutarate semialdehyde + NADH | - |
? | |
glutarate semialdehyde + NAD+ + H2O | - |
Pseudomonas putida | glutarate + NADH | - |
? | |
glutarate semialdehyde + NAD+ + H2O | constitutive levels of the enzyme are produced under all growth conditions. Growth on Lys leads to specific activity levels 2-3fold higher than growth on other amino acids | Pseudomonas putida | glutarate + NADH | - |
? | |
heptaldehyde + NAD+ + H2O | low activity | Pseudomonas putida | heptanoate + NADH | - |
? | |
valeraldehyde + NAD+ + H2O | low activity | Pseudomonas putida | valeric acid + NADH + H+ | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | coenzyme | Pseudomonas putida |