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Literature summary for 1.2.1.19 extracted from

  • Testore, G.; Colombatto, S.; Silvagno, F.; Bedino, S.
    Purification and kinetic characterization of gamma-aminobutyraldehyde dehydrogenase from rat liver (1995), Int. J. Biochem. Cell Biol., 27, 1201-1210.
    View publication on PubMed
Search for more literature for 1.2.1.19

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol 4-8 mM, full reactivation after inactivation due to overnight dialysis against 50 mM phosphate pH 7 Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
acetaldehyde strongly inhibits 4-aminobutyraldehyde oxidation Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.003
-
 capronaldehyde same value with benzaldehyde, constant substrate 1 mM NAD+ Rattus norvegicus
0.004
-
 Valeraldehyde constant substrate 1 mM NAD+ Rattus norvegicus
0.005
-
 Butyraldehyde constant substrate 1 mM NAD+ Rattus norvegicus
0.013
-
 propionaldehyde constant substrate 1 mM NAD+ Rattus norvegicus
0.016
-
 Isobutyraldehyde constant substrate 1 mM NAD+ Rattus norvegicus
0.07
-
 NAD+ constant substrate 2 mM 4-aminobutyraldehyde Rattus norvegicus
0.109
-
 4-Aminobutyraldehyde constant substrate 1 mM NAD+ Rattus norvegicus
0.111
-
 acetaldehyde constant substrate 1 mM NAD+ Rattus norvegicus
0.186
-
 NAD+ constant substrate 0.1 mM capronaldehyde Rattus norvegicus
0.256
-
 NAD+ constant substrate 0.2 mM isotbutyraldehyde Rattus norvegicus
0.886
-
 NAD+ constant substrate 2 mM acetaldehyde Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Rattus norvegicus 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 10% activation by 2 mM observed with 0.1 mM capronaldehyde as substrate Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
51000
-
 2 * 51000, SDS-PAGE Rattus norvegicus
115000
-
 gel filtration Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
 Wistar-strain
-
Rattus norvegicus Wistar-
-
 Wistar-strain
-

Purification (Commentary)

Purification (Comment) Organism
using column chromatography on DEAE-cellulose, hydroxylapatite, Sephacryl S-300 and Phenyl-Superose and isoelectrofocusing Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Rattus norvegicus

Storage Stability

Storage Stability Organism
4°C, 250 mM phosphate, pH 6.8, in the presence of 4 mM dithiothreitol, activity decreases by 20-30% after 7 days, freezing resultes in complete inactivation Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-aminobutanal + NAD+ + H2O
-
 Rattus norvegicus 4-aminobutanoate + NADH + H+
-
 ?
4-aminobutanal + NAD+ + H2O
-
 Rattus norvegicus Wistar- 4-aminobutanoate + NADH + H+
-
 ?
acetaldehyde + NAD+ + H2O
-
 Rattus norvegicus acetate + NADH + H+
-
 ?
benzaldehyde + NAD+ + H2O
-
 Rattus norvegicus benzoate + NADH + H+
-
 ?
butyraldehyde + NAD+ + H2O
-
 Rattus norvegicus butyrate + NADH + H+
-
 ?
butyraldehyde + NAD+ + H2O
-
 Rattus norvegicus Wistar- butyrate + NADH + H+
-
 ?
capronaldehyde + NAD+ + H2O
-
 Rattus norvegicus capronate + NADH + H+
-
 ?
capronaldehyde + NAD+ + H2O
-
 Rattus norvegicus Wistar- capronate + NADH + H+
-
 ?
isobutyraldehyde + NAD+ + H2O
-
 Rattus norvegicus isobutyrate + NADH + H+
-
 ?
propionaldehyde + NAD+ + H2O
-
 Rattus norvegicus propionate + NADH + H+
-
 ?
propionaldehyde + NAD+ + H2O
-
 Rattus norvegicus Wistar- propionate + NADH + H+
-
 ?
valeraldehyde + NAD+ + H2O
-
 Rattus norvegicus valerate + NADH + H+
-
 ?
valeraldehyde + NAD+ + H2O
-
 Rattus norvegicus Wistar- valerate + NADH + H+
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 51000, SDS-PAGE Rattus norvegicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
28
-
 temperature higher than 28°C promotes slow activation Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3 8.4
-
 Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Rattus norvegicus