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Literature summary for 1.2.1.16 extracted from

  • Ito, F.; Chishiki, H.; Fushinobu, S.; Wakagi, T.
    Archaeal aldehyde dehydrogenase ST0064 from Sulfolobus tokodaii, a paralog of non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase, is a succinate semialdehyde dehydrogenase (2013), Biosci. Biotechnol. Biochem., 77, 1344-1348.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Sulfurisphaera tokodaii

Protein Variants

Protein Variants Comment Organism
S434A kinetic data show no significant difference from wild-type enzyme Sulfurisphaera tokodaii
S434D kinetic data show no significant difference from wild-type enzyme Sulfurisphaera tokodaii
S434E kinetic data show no significant difference from wild-type enzyme Sulfurisphaera tokodaii

Inhibitors

Inhibitors Comment Organism Structure
coenzyme A 1 mM, 15% inhibition Sulfurisphaera tokodaii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.002
-
succinate semialdehyde pH 8.0, 60°C, cosubstrate: NAD+ Sulfurisphaera tokodaii
0.0029
-
succinate semialdehyde pH 8.0, 60°C, cosubstrate: NADP+ Sulfurisphaera tokodaii
0.043
-
NADP+ pH 8.0, 60°C Sulfurisphaera tokodaii
3.3
-
NAD+ pH 8.0, 60°C Sulfurisphaera tokodaii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000
-
4 * 52000, SDS-PAGE Sulfurisphaera tokodaii
180000
-
gel filtration Sulfurisphaera tokodaii

Organism

Organism UniProt Comment Textmining
Sulfurisphaera tokodaii Q976X5
-
-
Sulfurisphaera tokodaii 7 Q976X5
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information neither propionaldehyde nor phthalaldehyde seves as substrate Sulfurisphaera tokodaii ?
-
?
additional information neither propionaldehyde nor phthalaldehyde seves as substrate Sulfurisphaera tokodaii 7 ?
-
?
succinate semialdehyde + NAD+ + H2O
-
Sulfurisphaera tokodaii succinate + NADH + 2 H+
-
?
succinate semialdehyde + NAD+ + H2O
-
Sulfurisphaera tokodaii 7 succinate + NADH + 2 H+
-
?
succinate semialdehyde + NADP+ + H2O
-
Sulfurisphaera tokodaii succinate + NADPH + 2 H+
-
?
succinate semialdehyde + NADP+ + H2O
-
Sulfurisphaera tokodaii 7 succinate + NADPH + 2 H+
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 52000, SDS-PAGE Sulfurisphaera tokodaii

Synonyms

Synonyms Comment Organism
ST0064
-
Sulfurisphaera tokodaii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
90
-
-
Sulfurisphaera tokodaii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
27.6
-
NADP+ pH 8.0, 60°C Sulfurisphaera tokodaii
29.4
-
succinate semialdehyde pH 8.0, 40°C, cosubstrate: NAD+ Sulfurisphaera tokodaii
30
-
succinate semialdehyde pH 8.0, 40°C, cosubstrate: NADP+ Sulfurisphaera tokodaii
51.2
-
NAD+ pH 8.0, 60°C Sulfurisphaera tokodaii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Sulfurisphaera tokodaii

Cofactor

Cofactor Comment Organism Structure
NAD+ kcat/Km-value is comparable for succinate semialdehyde with NADP+ and for succinate semialdehyde with NAD+ Sulfurisphaera tokodaii
NADP+ kcat/Km-value is comparable for succinate semialdehyde with NADP+ and for succinate semialdehyde with NAD+ Sulfurisphaera tokodaii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
15.5
-
NAD+ pH 8.0, 60°C Sulfurisphaera tokodaii
643
-
NADP+ pH 8.0, 60°C Sulfurisphaera tokodaii
10300
-
succinate semialdehyde pH 8.0, 60°C, cosubstrate: NADP+ Sulfurisphaera tokodaii
14700
-
succinate semialdehyde pH 8.0, 60°C, cosubstrate: NAD+ Sulfurisphaera tokodaii