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Literature summary for 1.2.1.13 extracted from
- The phosphoglycerate kinase and glyceraldehyde-3-phosphate dehydrogenase genes from the thermophilic archaeon Sulfolobus solfataricus overlap by 8-bp. Isolation, sequencing of the genes and expression in Escherichia coli (1995), Eur. J. Biochem., 233, 800-808.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli. The phosphoglycerate kinase and glyceraldehyde-3-phosphate dehydrogenase genes from Sulfolobus solfataricus overlap by 8-bp | Saccharolobus solfataricus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.067 | - |
NADP+ | pH 7.5, 50°C | Saccharolobus solfataricus |  |
| 2.2 | - |
NAD+ | pH 7.5, 50°C | Saccharolobus solfataricus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 39500 | - |
x * 39500, SDS-PAGE | Saccharolobus solfataricus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharolobus solfataricus | P39460 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| heat treatment at 80°C is an effective first step in the purification of these recombinant enzymes from extracts of the Escherichia coli host | Saccharolobus solfataricus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4.97 | - |
pH 7.5, 50°C | Saccharolobus solfataricus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glyceraldehyde 3-phosphate + phosphate + NAD+ | Vmax/Km for NADP+ is 33fold higher compared to Vmax/Km for NAD+ | Saccharolobus solfataricus | 3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
? | |
| D-glyceraldehyde 3-phosphate + phosphate + NADP+ | Vmax/Km for NADP+ is 33fold higher compared to Vmax/Km for NAD+ | Saccharolobus solfataricus | 3-phospho-D-glyceroyl phosphate + NADPH + H+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 39500, SDS-PAGE | Saccharolobus solfataricus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| glyceraldehyde-3-phosphate dehydrogenase | - |
Saccharolobus solfataricus |
| GraP-DH | - |
Saccharolobus solfataricus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
assay at | Saccharolobus solfataricus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
half life: 17 h | Saccharolobus solfataricus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Saccharolobus solfataricus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | Vmax/Km for NADP+ is 33fold higher compared to Vmax/Km for NAD+ | Saccharolobus solfataricus | |
| NADP+ | Vmax/Km for NADP+ is 33fold higher compared to Vmax/Km for NAD+ | Saccharolobus solfataricus | |
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