Inhibitors | Comment | Organism | Structure |
---|---|---|---|
iodoacetate | preincubation with glyceraldehyde-phosphate, 10fold molar excess over iodoacetate, prevents inactivation | Spinacia oleracea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Spinacia oleracea |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Spinacia oleracea | 9507 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
x * 43000 + x * 37000, SDS-PAGE | Spinacia oleracea |
43000 | - |
x * 43000 + x * 37000, SDS-PAGE | Spinacia oleracea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Spinacia oleracea | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Spinacia oleracea |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
13.92 | - |
NAD+-dependent activity | Spinacia oleracea |
15.4 | - |
NADP+-dependent activity | Spinacia oleracea |
Storage Stability | Organism |
---|---|
4°C, pH 8.5, protein concentration 2.5 mg/ml, apoprotein, complete loss of activity after 48 h | Spinacia oleracea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Spinacia oleracea | 3-phospho-D-glyceroyl phosphate + NADH | - |
r | |
D-glyceraldehyde 3-phosphate + phosphate + NADP+ | - |
Spinacia oleracea | 3-phospho-D-glyceroyl phosphate + NADPH | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 43000 + x * 37000, SDS-PAGE | Spinacia oleracea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 8.8 | both directions | Spinacia oleracea |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | cofactor | Spinacia oleracea |