Cloned (Comment) | Organism |
---|---|
recombinant expression of enzyme gapcp double mutants, gapcp1gapcp2, under the control of photosynthetic (Rubisco small subunit RBCS2B [RBCS]) or heterotrophic (phosphate transporter PHT1.2 [PHT]) cell-specific promoters. Expression of GAPCp1 under the control of RBCS in gapcp1gapcp2 has no significant effect on the metabolite profile or growth in the aerial part (AP). GAPCp1 expression under the control of the PHT promoter clearly affects Arabidopsis thaliana development by increasing the number of lateral roots and having a major effect on AP growth and metabolite profile. Recombinant expression of GFP-tagged isozyme GAPCp1 in Arabidopsis thaliana, quantitative real-time-PCR expression analysis | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of enzyme gapcp double mutants, gapcp1gapcp2, under the control of photosynthetic (Rubisco small subunit RBCS2B [RBCS]) or heterotrophic (phosphate transporter PHT1.2 [PHT]) cell-specific promoters | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Arabidopsis thaliana | 9507 | - |
chloroplast | plastidial glycolytic isoform 2 of glyceraldehyde-3-phosphate dehydrogenase, GAPCp2 | Arabidopsis thaliana | 9507 | - |
plastid | plastidial glycolytic isoform 1 of glyceraldehyde-3-phosphate dehydrogenase, GAPCp1 | Arabidopsis thaliana | 9536 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | Arabidopsis thaliana | - |
3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q5E924 | - |
- |
Arabidopsis thaliana | Q9SAJ6 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
aerial part | - |
Arabidopsis thaliana | - |
leaf | - |
Arabidopsis thaliana | - |
meristem | - |
Arabidopsis thaliana | - |
root | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Arabidopsis thaliana | 3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GAPCp1 | - |
Arabidopsis thaliana |
GAPCp2 | - |
Arabidopsis thaliana |
glyceraldehyde-3-phosphate dehydrogenase | - |
Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
malfunction | transcriptomic and metabolomic analyses indicate that the lack of GAPCp activity affects nitrogen and carbon metabolism as well as mineral nutrition and that glycerate and glutamine are the main metabolites responding to GAPCp activity, phenotypic analysis, detailed overview. Mutants gapcp1gapcp2 display a drastic reduction not only of root growth but also of the aerial part (AP) when grown both on plates and in greenhouse conditions. This phenotype is observed in double homozygous mutants only. Single mutants (gapcp1 or gapcp2) or mutant plants homozygous for one of the genes and heterozygous for the other are phenotypically indistinguishable from the wild-type plants. At the adult stage, GAPCp1 expression in the AP is able to complement the sterile phenotype of gapcp1gapcp2, resulting in plants with siliques and fertile seeds. The developmental pattern of gapcp1gapcp2 RBCS:GAPCp1 is also altered as compared with gapcp1gapcp2, probably as a consequence of the fertile phenotype, displaying shorter shoots than gapcp1gapcp2. A similar developmental pattern alteration is observed in the sterile gapcp1gapcp2 35S:GAPCp1 lines | Arabidopsis thaliana |
malfunction | transcriptomic and metabolomic analyses indicate that the lack of GAPCp activity affects nitrogen and carbon metabolism as well as mineral nutrition and that glycerate and glutamine are the main metabolites responding to GAPCp activity, phenotypic analysis, detailed overview. Mutants gapcp1gapcp2 display a drastic reduction not only of root growth but also of the aerial part when grown both on plates and in greenhouse conditions. This phenotype is observed in double homozygous mutants only. Single mutants (gapcp1 or gapcp2) or mutant plants homozygous for one of the genes and heterozygous for the other are phenotypically indistinguishable from the wild-type plants | Arabidopsis thaliana |
metabolism | GAPCp might be an important metabolic connector of glycolysis with other pathways, such as the phosphorylated pathway of serine biosynthesis, the ammonium assimilation pathway, or the metabolism of gamma-aminobutyrate, which in turn affect plant development | Arabidopsis thaliana |
metabolism | plastidial isozyme GAPCp might be an important metabolic connector of glycolysis with other pathways, such as the phosphorylated pathway of serine biosynthesis, the ammonium assimilation pathway, or the metabolism of gamma-aminobutyrate, which in turn affect plant development | Arabidopsis thaliana |
physiological function | plastidial isozymes GAPCps are critical for primary root growth and essential for microspore development. Plastidial isozyme GAPCp1 is not functionally important in photosynthetic cells but plays a fundamental role in roots and in heterotrophic cells of the aerial part. GAPCp1 expression in reproductive organs is necessary for Arabidopsis fertility. GAPCp activity may be required in root meristems and the root cap for normal primary root growth. GAPCp might be an important metabolic connector of glycolysis with other pathways, such as the phosphorylated pathway of serine biosynthesis, the ammonium assimilation pathway, or the metabolism of gamma-aminobutyrate, which in turn affect plant development. Isozymes GAPCp1 and GAPCp2 are redundant to one another | Arabidopsis thaliana |
physiological function | the plastidial GAPCps are critical for primary root growth and essential for microspore development. Plastidial isozyme GAPCp might be an important metabolic connector of glycolysis with other pathways, such as the phosphorylated pathway of serine biosynthesis, the ammonium assimilation pathway, or the metabolism of gamma-aminobutyrate, which in turn affect plant development. Isozymes GAPCp1 and GAPCp2 are redundant to one another | Arabidopsis thaliana |