Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli M15 cells | Staphylococcus aureus |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 0.1 M Tris-HCl pH 8.2, 28% (w/v) PEG 3350, at 25°C | Staphylococcus aureus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | Staphylococcus aureus | - |
3-phospho-D-glyceroyl phosphate + NADH | - |
? | |
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | Staphylococcus aureus MRSA252 | - |
3-phospho-D-glyceroyl phosphate + NADH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | Q6GIL8 | - |
- |
Staphylococcus aureus MRSA252 | Q6GIL8 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-Sepharose column chromatography and Superdex 75 gel filtration | Staphylococcus aureus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Staphylococcus aureus | 3-phospho-D-glyceroyl phosphate + NADH | - |
? | |
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Staphylococcus aureus MRSA252 | 3-phospho-D-glyceroyl phosphate + NADH | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | x-ray crystallography | Staphylococcus aureus |
Synonyms | Comment | Organism |
---|---|---|
GAP1 | - |
Staphylococcus aureus |
glyceraldehyde-3-phosphate dehydrogenase 1 | - |
Staphylococcus aureus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Staphylococcus aureus |