Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)pLysS cells | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
C159S | the mutant C159S of the isozyme GapC2 shows decreased specific activity | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
glutathione | inactivation with 10 mM glutathione is reversible upon addition of 20 mM dithiothreitol | Arabidopsis thaliana | |
glutathione | inactivation with 10 mM glutathione is reversible upon addition of 20 mM dithiothreitol | Oryctolagus cuniculus | |
glutathione | inactivation with 10 mM glutathione is reversible upon addition of 20 mM dithiothreitol | Saccharomyces cerevisiae | |
glutathione | inactivation with 10 mM glutathione is reversible upon addition of 20 mM dithiothreitol | Spinacia oleracea | |
oxidized glutathione | inactivation, at least partially reversible upon addition of dithiothreitol. Both residues C155 and C159 are found glutathionylated; inactivation, at least partially reversible upon addition of dithiothreitol. Both residues C155 and C159 are found glutathionylated | Arabidopsis thaliana | |
oxidized glutathione | inactivation, at least partially reversible upon addition of dithiothreitol | Oryctolagus cuniculus | |
oxidized glutathione | inactivation, at least partially reversible upon addition of dithiothreitol | Spinacia oleracea | |
S-nitrosoglutathione | inactivation, at least partially reversible upon addition of dithiothreitol. Both residues C155 and C159 are found nitrosylated; inactivation, at least partially reversible upon addition of dithiothreitol. Both residues C155 and C159 are found nitrosylated; inactivation with 0.5 mM S-nitrosoglutathione is reversible upon addition of 20 mM dithiothreitol | Arabidopsis thaliana | |
S-nitrosoglutathione | inactivation, at least partially reversible upon addition of dithiothreitol; inactivation with 0.5 mM S-nitrosoglutathione is reversible upon addition of 20 mM dithiothreitol | Oryctolagus cuniculus | |
S-nitrosoglutathione | inactivation with 0.5 mM S-nitrosoglutathione is reversible upon addition of 20 mM dithiothreitol | Saccharomyces cerevisiae | |
S-nitrosoglutathione | inactivation, at least partially reversible upon addition of dithiothreitol; inactivation with 0.5 mM S-nitrosoglutathione is reversible upon addition of 20 mM dithiothreitol | Spinacia oleracea |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Saccharomyces cerevisiae | 5829 | - |
cytosol | - |
Oryctolagus cuniculus | 5829 | - |
cytosol | - |
Spinacia oleracea | 5829 | - |
cytosol | - |
Arabidopsis thaliana | 5829 | - |
nucleus | - |
Arabidopsis thaliana | 5634 | - |
nucleus | of protoplast, both isoforms GapC1 and GapC2 | Arabidopsis thaliana | 5634 | - |
protoplast | - |
Arabidopsis thaliana | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | P25858 | ecotype Columbia | - |
Arabidopsis thaliana | P25858 | isoform GapC1 | - |
Arabidopsis thaliana | Q56WJ4 | isoform GapC2 | - |
Oryctolagus cuniculus | - |
- |
- |
Saccharomyces cerevisiae | - |
- |
- |
Spinacia oleracea | - |
- |
- |
Oxidation Stability | Organism |
---|---|
stable after reactivation and desalting for at least 2 h when incubated in the presence of 20 mM dithiothreitol, but less stable when both, NAD+ and the reductant, are omitted in the pre-incubation assays, under this condition the enzymes seem to be very sensitive towards traces of oxygen in the media, but upon addition of 20 mM dithiothritol after 1 h, the full activity is restored | Saccharomyces cerevisiae |
stable after reactivation and desalting for at least 2 h when incubated in the presence of 20 mM dithiothreitol, but less stable when both, NAD+ and the reductant, are omitted in the pre-incubation assays, under this condition the enzymes seem to be very sensitive towards traces of oxygen in the media, but upon addition of 20 mM dithiothritol after 1 h, the full activity is restored | Oryctolagus cuniculus |
stable after reactivation and desalting for at least 2 h when incubated in the presence of 20 mM dithiothreitol, but less stable when both, NAD+ and the reductant, are omitted in the pre-incubation assays, under this condition the enzymes seem to be very sensitive towards traces of oxygen in the media, but upon addition of 20 mM dithiothritol after 1 h, the full activity is restored | Spinacia oleracea |
stable after reactivation and desalting for at least 2 h when incubated in the presence of 20 mM dithiothreitol, but less stable when both, NAD+ and the reductant, are omitted in the pre-incubation assays, under this condition the enzymes seem to be very sensitive towards traces of oxygen in the media, but upon addition of 20 mM dithiothritol after 1 h, the full activity is restored | Arabidopsis thaliana |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation and Q-Sepharose column chromatography | Saccharomyces cerevisiae |
ammonium sulfate precipitation and Q-Sepharose column chromatography | Oryctolagus cuniculus |
ammonium sulfate precipitation and Q-Sepharose column chromatography | Spinacia oleracea |
ammonium sulfate precipitation and Q-Sepharose column chromatography | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | - |
Arabidopsis thaliana | - |
leaf | - |
Spinacia oleracea | - |
leaf | - |
Arabidopsis thaliana | - |
muscle | - |
Oryctolagus cuniculus | - |
silique | - |
Arabidopsis thaliana | - |
stem | - |
Arabidopsis thaliana | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
30 | - |
purified isozyme GapC1 | Arabidopsis thaliana |
33 | - |
purified GapC2 C159S mutant isozyme | Arabidopsis thaliana |
60 | - |
purified isozyme GapC2 | Arabidopsis thaliana |
90 | - |
purified enzyme | Spinacia oleracea |
100 | - |
purified enzyme | Saccharomyces cerevisiae |
100 | - |
purified enzyme | Oryctolagus cuniculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Saccharomyces cerevisiae | 3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
? | |
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Oryctolagus cuniculus | 3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
? | |
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Spinacia oleracea | 3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
? | |
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Arabidopsis thaliana | 3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | enzyme can bind top a partial gene sequence of NADP-dependent malated dehydrogenase EC 1.2.1.37 | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
cytosolic NAD-dependent glyceraldehyde 3-P dehydrogenase | - |
Saccharomyces cerevisiae |
cytosolic NAD-dependent glyceraldehyde 3-P dehydrogenase | - |
Oryctolagus cuniculus |
cytosolic NAD-dependent glyceraldehyde 3-P dehydrogenase | - |
Spinacia oleracea |
cytosolic NAD-dependent glyceraldehyde 3-P dehydrogenase | - |
Arabidopsis thaliana |
GapC1 | isozyme | Arabidopsis thaliana |
GapC2 | isozyme | Arabidopsis thaliana |
GAPDH | - |
Saccharomyces cerevisiae |
GAPDH | - |
Oryctolagus cuniculus |
GAPDH | - |
Spinacia oleracea |
GAPDH | - |
Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Saccharomyces cerevisiae | |
NAD+ | - |
Oryctolagus cuniculus | |
NAD+ | - |
Spinacia oleracea | |
NAD+ | - |
Arabidopsis thaliana |