Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AMP | 18 mM, only 5% loss of activity | Dissostichus mawsoni | |
ATP | 1 mM, 25% loss of activity | Dissostichus mawsoni | |
D-glyceraldehyde 3-phosphate | substrate inhibition | Dissostichus mawsoni |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.018 | - |
NAD+ | - |
Oryctolagus cuniculus | |
0.0435 | - |
thio-NAD+ | - |
Oryctolagus cuniculus | |
0.076 | - |
NAD+ | - |
Dissostichus mawsoni | |
0.158 | - |
3-acetyl-NAD+ | - |
Oryctolagus cuniculus | |
0.23 | - |
D-glyceraldehyde 3-phosphate | - |
Dissostichus mawsoni | |
0.235 | - |
thio-NAD+ | - |
Dissostichus mawsoni | |
0.62 | - |
3-acetyl-NAD+ | - |
Dissostichus mawsoni | |
1.43 | - |
3-acetylpyridine hypoxanthine nucleotide | - |
Oryctolagus cuniculus | |
8.15 | - |
3-acetylpyridine hypoxanthine nucleotide | - |
Dissostichus mawsoni |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
142000 | - |
low speed equilibrium sedimentation | Dissostichus mawsoni |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dissostichus mawsoni | - |
- |
- |
Oryctolagus cuniculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Dissostichus mawsoni |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
muscle | - |
Oryctolagus cuniculus | - |
muscle | skeletal muscle | Dissostichus mawsoni | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate + 3-acetylpyridine hypoxanthine nucleotide + phosphate | 1.3% of the activity with NAD+ | Dissostichus mawsoni | 3-phospho-D-glyceroyl phosphate + ? | - |
? | |
D-glyceraldehyde 3-phosphate + 3-acetylpyridine hypoxanthine nucleotide + phosphate | 3.2% of activity with NAD+ | Oryctolagus cuniculus | 3-phospho-D-glyceroyl phosphate + ? | - |
? | |
D-glyceraldehyde 3-phosphate + 3-acetylpyridine NAD+ + phosphate | 2.8% of the activity with NAD+ | Dissostichus mawsoni | 3-phospho-D-glyceroyl phosphate + ? | - |
? | |
D-glyceraldehyde 3-phosphate + 3-acetylpyridine NAD+ + phosphate | 4.5% of activity with NAD+ | Oryctolagus cuniculus | 3-phospho-D-glyceroyl phosphate + ? | - |
? | |
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Oryctolagus cuniculus | 3-phospho-D-glyceroyl phosphate + NADH | - |
? | |
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Dissostichus mawsoni | 3-phospho-D-glyceroyl phosphate + NADH | - |
? | |
D-glyceraldehyde 3-phosphate + thio-NAD+ + phosphate | 7.2% of the activity with NAD+ | Dissostichus mawsoni | 3-phospho-D-glyceroyl phosphate + thio-NADH | - |
? | |
D-glyceraldehyde 3-phosphate + thio-NAD+ + phosphate | 9.3% of activity with NAD+ | Oryctolagus cuniculus | 3-phospho-D-glyceroyl phosphate + thio-NADH | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
30 min, stable up to | Dissostichus mawsoni |
55 | - |
30 min, 50% loss of activity | Dissostichus mawsoni |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
3-acetylpyridine hypoxanthine nucleotide | 3.2% of activity with NAD+ | Oryctolagus cuniculus | |
3-acetylpyridine hypoxanthine nucleotide | 1.3% of the activity with NAD+ | Dissostichus mawsoni | |
3-acetylpyridine-NAD+ | 4.5% of activity with NAD+ as cofactor | Oryctolagus cuniculus | |
3-acetylpyridine-NAD+ | 2.8% of the activity with NAD+ | Dissostichus mawsoni | |
NAD+ | - |
Oryctolagus cuniculus | |
NAD+ | contains 4 mol of NAD+ per mol of enzyme | Dissostichus mawsoni | |
thio-NAD+ | 9.3% of activity with NAD+ | Oryctolagus cuniculus | |
thio-NAD+ | 7.2% of the activity with NAD+ | Dissostichus mawsoni |