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Literature summary for 1.2.1.12 extracted from
- Purification and characterization of thermolabile glyceraldehyde-3-phosphate dehydrogenase from the facultative thermophile Bacillus coagulans KU (1977), Biochemistry, 16, 4840-4847.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Weizmannia coagulans |
General Stability
| General Stability | Organism |
|---|---|
| 8 M urea, 30°C, 0.05 M phosphate buffer, 50% loss of activity after 1 min | Weizmannia coagulans |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36000 | - |
4 * 36000, SDS-PAGE | Weizmannia coagulans |
| 146800 | - |
equilibrium sedimentation | Weizmannia coagulans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Weizmannia coagulans | - |
KU | - |
| Weizmannia coagulans KU | - |
KU | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Weizmannia coagulans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 109 | - |
- |
Weizmannia coagulans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Weizmannia coagulans | 3-phospho-D-glyceroyl phosphate + NADH | - |
? |  |
| D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Weizmannia coagulans KU | 3-phospho-D-glyceroyl phosphate + NADH | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 36000, SDS-PAGE | Weizmannia coagulans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
5 min, 96-98% loss of activity. No inactivation if the ionic strength is increased to 1.8 by addition of NaCl or (NH4)2SO4 | Weizmannia coagulans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.6 | - |
- |
Weizmannia coagulans |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | enzyme contains 4 equivalents of firmly bound NAD+ | Weizmannia coagulans | |
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