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Literature summary for 1.2.1.12 extracted from
- Glyceraldehyde-3-phosphate dehydrogenase from Thermus thermophilus (1982), Methods Enzymol., 89, 335-340.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| acetone | activates | Thermus thermophilus |  |
| ethanol | 30% v/v, 5fold activation | Thermus thermophilus | |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Thermus thermophilus |
General Stability
| General Stability | Organism |
|---|---|
| 1% SDS, fully active | Thermus thermophilus |
| 8 M urea, 40% inactivation | Thermus thermophilus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CdCl2 | - |
Thermus thermophilus | |
| Cu2+ | - |
Thermus thermophilus | |
| HgCl2 | - |
Thermus thermophilus | |
| iodoacetamide | - |
Thermus thermophilus | |
| iodoacetate | - |
Thermus thermophilus | |
| Na2S4O6 | - |
Thermus thermophilus | |
| Sodium tetrathionate | - |
Thermus thermophilus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.1 | - |
NAD+ | - |
Thermus thermophilus | |
| 0.3 | - |
D-glyceraldehyde 3-phosphate | - |
Thermus thermophilus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| (NH4)2SO4 | 0.045 M, 10fold activation | Thermus thermophilus | |
| CsCl | activates | Thermus thermophilus | |
| K+ | KCl, activates | Thermus thermophilus | |
| LiCl | activates | Thermus thermophilus | |
| Na2SO4 | up to 5fold activation | Thermus thermophilus | |
| NaCl | up to 5fold activation | Thermus thermophilus | |
| NH4Cl | 0.1 M, 25fold activation | Thermus thermophilus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33500 | - |
4 * 33500, SDS-PAGE | Thermus thermophilus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermus thermophilus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermus thermophilus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 5.4 | - |
crystalline enzyme | Thermus thermophilus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, stable for at least 2 years | Thermus thermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glyceraldehyde 3-phosphate + arsenate + NAD+ | the product arseno-3-phosphoglycerate is decomposed readily to 3-phosphoglycerate | Thermus thermophilus | 3-phospho-D-glyceroyl arsenate + NADH | - |
ir | |
| D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Thermus thermophilus | 3-phospho-D-glyceroyl phosphate + NADH | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 33500, SDS-PAGE | Thermus thermophilus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 90 | - |
slow inactivation | Thermus thermophilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.3 | - |
- |
Thermus thermophilus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Thermus thermophilus | |
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Release 2023.1 (January 2023)
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