KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
3-phospho-D-glyceroyl phosphate | enzyme form E6.8, pH 7 | Homo sapiens | |
0.01 | - |
NAD+ | enzyme form E8.5, pH 9 | Homo sapiens | |
0.01 | - |
1,3-diphosphoglyceric acid | enzyme form E6.6, pH 7 | Homo sapiens | |
0.012 | - |
1,3-diphosphoglyceric acid | enzyme form E8.5, pH 7 | Homo sapiens | |
0.018 | - |
1,3-diphosphoglyceric acid | enzyme form E9.0, pH 7 | Homo sapiens | |
0.02 | - |
NAD+ | enzyme form E9.0, pH 9 | Homo sapiens | |
0.027 | - |
NAD+ | enzyme form E6.8, pH 9 | Homo sapiens | |
0.032 | - |
3-phospho-D-glyceroyl phosphate | enzyme form E9.0, pH 9 | Homo sapiens | |
0.035 | - |
3-phospho-D-glyceroyl phosphate | enzyme form E8.5, pH 9 | Homo sapiens | |
0.042 | - |
3-phospho-D-glyceroyl phosphate | enzyme form E6.6, pH 9 | Homo sapiens | |
0.1 | - |
DL-glyceraldehyde | enzyme form E8.5, pH 7 | Homo sapiens | |
0.119 | - |
erythrose 4-phosphate | enzyme form E8.5, pH 7 | Homo sapiens | |
0.127 | - |
erythrose 4-phosphate | enzyme form E9.0, pH 7 | Homo sapiens | |
0.143 | - |
NAD+ | enzyme form E6.6, pH 9 | Homo sapiens | |
0.172 | - |
3-phospho-D-glyceroyl phosphate | enzyme form E6.8, pH 9 | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Rattus norvegicus | 5739 | - |
mitochondrion | enzyme form E6.8, E8.5 and E9.0 | Homo sapiens | 5739 | - |
additional information | the subcellular localization of enzyme form E6.6 is uncertain | Homo sapiens | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn | enzyme form E8.5 contains 0.64 gatoms of zinc per mol of enzyme, enzyme form E9.0 contain 2.76 gatom of zinc per mol of enzyme | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29500 | - |
4 * 29500, enzyme form E8.5, SDS-PAGE | Homo sapiens |
33000 | - |
4 * 33000, enzyme form E9.5, SDS-PAGE | Homo sapiens |
36000 | - |
x * 36000 + x * 38000, enzyme form E6.8, SDS-PAGE | Homo sapiens |
38000 | - |
x * 36000 + x * 38000, enzyme form E6.8, SDS-PAGE | Homo sapiens |
58000 | - |
1 * 58000 + 1 * 61000, enzyme form E6.6, SDS-PAGE | Homo sapiens |
61000 | - |
1 * 58000 + 1 * 61000, enzyme form E6.6, SDS-PAGE | Homo sapiens |
98000 | - |
enzyme form E6.6, gel filtration | Homo sapiens |
120000 | - |
enzyme form E8.5, gel filtration | Homo sapiens |
133000 | - |
enzyme form E9.0, gel filtration | Homo sapiens |
142000 | - |
enzyme form E6.8, gel filtration | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
4 enzyme forms: E6.6, E6.8, E8.5 and E9.0 | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Homo sapiens | - |
liver | - |
Homo sapiens | - |
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
8.6 | - |
enzyme form E6.6 | Homo sapiens |
13 | - |
enzyme form E6.8 | Homo sapiens |
158 | - |
enzyme form E8.5 | Homo sapiens |
620 | - |
enzyme form E9.0 | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-phospho-D-glyceroyl phosphate + NADH | - |
Homo sapiens | D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
r | |
acetaldehyde + phosphate + NAD+ | enzyme form E6.6 shows 27% of the activity with D-glyceraldehyde 3-phosphate, enzyme form E6.8 shows 9% of the activity with D-glyceraldehyde 3-phosphate, enzyme form E8.5 shows 6% of the actity with D-glyceraldehyde 3-phosphate, enzyme form E9.0 shows 0.4% of the activity with D-glyceraldehyde 3-phosphate | Homo sapiens | acetyl phosphate + NADH | - |
? | |
butyraldehyde + phosphate + NAD+ | enzyme form E6.6 shows 10% of the activity with D-glyceraldehyde 3-phosphate, enzyme form E6.8 shows 15% of the activity with D-glyceraldehyde 3-phosphate, enzyme form E8.5 shows 12% of the actity with D-glyceraldehyde 3-phosphate, enzyme form E9.0 shows 0.9% of the activity with D-glyceraldehyde 3-phosphate | Homo sapiens | butyryl phosphate + NADH | - |
? | |
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Homo sapiens | 3-phospho-D-glyceroyl phosphate + NADH | - |
r | |
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Rattus norvegicus | 3-phospho-D-glyceroyl phosphate + NADH | - |
? | |
DL-glyceraldehyde + phosphate + NAD+ | enzyme form E6.6 shows no activity, enzyme form E6.8 shows 2.5% of the activity with D-glyceraldehyde 3-phosphate, enzyme form E8.5 shows 30% of the actity with D-glyceraldehyde 3-phosphate, enzyme form E9.0 shows 3.0% of the activity with D-glyceraldehyde 3-phosphate | Homo sapiens | D-glyceroyl phosphate + NADH | - |
? | |
erythrose 4-phosphate + phosphate + NAD+ | enzyme form E6.6 shows no activity, enzyme form E6.8 shows 1.2% of the activity with D-glyceraldehyde 3-phosphate, enzyme form E8.5 shows 25% of the actity with D-glyceraldehyde 3-phosphate, enzyme form E9.0 shows 1.5% of the activity with D-glyceraldehyde 3-phosphate | Homo sapiens | ? + NADH | - |
? | |
glucose + phosphate + NAD+ | enzyme form E6.6 shows no activity, enzyme form E6.8 shows 0.6% of the activity with D-glyceraldehyde 3-phosphate, enzyme form E8.5 shows 6.0% of the actity with D-glyceraldehyde 3-phosphate, enzyme form E9.0 shows 0.8% of the activity with D-glyceraldehyde 3-phosphate | Homo sapiens | ? + NADH | - |
? | |
propionaldehyde + phosphate + NAD+ | enzyme form E6.6 shows 33% of the activity with D-glyceraldehyde 3-phosphate, enzyme form E6.8 shows 12% of the activity with D-glyceraldehyde 3-phosphate, enzyme form E8.5 shows 10% of the actity with D-glyceraldehyde 3-phosphate, enzyme form E9.0 shows 0.8% of the activity with D-glyceraldehyde 3-phosphate | Homo sapiens | propionyl phosphate + NADH | - |
? | |
valeraldehyde + phosphate + NAD+ | enzyme form E6.6 shows no activity, enzyme form E6.8 shows 19% of the activity with D-glyceraldehyde 3-phosphate, enzyme form E8.5 shows 18% of the actity with D-glyceraldehyde 3-phosphate, enzyme form E9.0 shows 0.9% of the activity with D-glyceraldehyde 3-phosphate | Homo sapiens | pentanoyl phosphate + NADH | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 58000 + 1 * 61000, enzyme form E6.6, SDS-PAGE | Homo sapiens |
tetramer | 4 * 29500, enzyme form E8.5, SDS-PAGE | Homo sapiens |
tetramer | x * 36000 + x * 38000, enzyme form E6.8, SDS-PAGE | Homo sapiens |
tetramer | 4 * 33000, enzyme form E9.5, SDS-PAGE | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
enzyme form E6.8, two pH optima: pH 7.0 and pH 8.5, with activity between pH 7.5 and pH 8.0 being rather low | Homo sapiens |
8.5 | - |
enzyme form E6.8, two pH optima: pH 7.0 and pH 8.5, with activity between pH 7.5 and pH 8.0 being rather low | Homo sapiens |
9.8 | - |
enzyme form E8.5, D-glyceraldehyde 3-phosphate | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Homo sapiens | |
NAD+ | - |
Rattus norvegicus | |
NADH | cofactor | Homo sapiens |