Literature summary for 1.2.1.11 extracted from

Zhang, W.W.; Jiang, W.H.; Zhao, G.P.; Yang, Y.L.; Chiao, J.S.
Expression in Escherichia coli, purification and kinetic analysis of the aspartokinase and aspartate semialdehyde dehydrogenase from the rifamycin SV-producing Amycolatopsis mediterranei U32 (2000), Appl. Microbiol. Biotechnol., 54, 52-58.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli	Amycolatopsis mediterranei U32

Inhibitors

Inhibitors	Comment	Organism	Structure
L-isoleucine	at 1 mM: 18% repression of enzyme synthesis, at 5 mM: 62% repression of enzyme synthesis	Amycolatopsis mediterranei U32
L-lysine	at 1 mM: 22% repression of the enzyme synthesis, at 5 mM: 44% repression of the enzyme synthesis, at 10 mM: 28% inhibition of the enzyme activity assayed in the reverse reaction	Amycolatopsis mediterranei U32
L-methionine	at 1 mM: 40% repression of the enzyme synthesis, at 5 mM: 62% repression of the enzyme synthesis	Amycolatopsis mediterranei U32
L-threonine	at 1 mM: 25% repression of the enzyme synthesis, at 5 mM: 43% repression of the enzyme synthesis, at 10 mM: 37% inhibition of the enzyme activity assayed in the reverse reaction	Amycolatopsis mediterranei U32

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.73	-	NADP+	-	Amycolatopsis mediterranei U32
1.25	-	L-aspartate 4-semialdehyde	-	Amycolatopsis mediterranei U32

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
37000	-	x * 37000, SDS-PAGE	Amycolatopsis mediterranei U32

Organism

Organism	UniProt	Comment	Textmining
Amycolatopsis mediterranei U32	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Amycolatopsis mediterranei U32

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.0029	-	of the recombinant in the crude extracts of Escherichia coli	Amycolatopsis mediterranei U32

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-aspartate 4-semialdehyde + phosphate + NADP+	-	Amycolatopsis mediterranei U32	L-4-aspartyl phosphate + NADPH	-	?

Subunits

Subunits	Comment	Organism
?	x * 37000, SDS-PAGE	Amycolatopsis mediterranei U32

Cofactor

Cofactor	Comment	Organism	Structure
NADP+	-	Amycolatopsis mediterranei U32

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Release 2023.1 (January 2023)