KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10.5 | - |
acetaldehyde | pH 7.0, 30°C | Citrobacter sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Citrobacter sp. | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
96000 | - |
4 * 96000, SDS-PAGE | Citrobacter sp. |
400000 | - |
gel filtration | Citrobacter sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Citrobacter sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ADHE cannot be solubilized from membrane with detergents such as 1% Triton X-100 or 1% sulfobetaine 3-12. The enzyme is easily dissociated from membrane by high-salt buffers containing either 1.0 M NaCl or (NH4)2SO4 without detergents | Citrobacter sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.11 | - |
cofactor NADP+, pH 7.0, 30°C | Citrobacter sp. |
0.47 | - |
cofactor NADP+, presence of CoA, pH 7.0, 30°C | Citrobacter sp. |
1.38 | - |
cofactor NAD+, pH 7.0, 30°C | Citrobacter sp. |
7.36 | - |
cofactor NAD+, presence of CoA, pH 7.0, 30°C | Citrobacter sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetaldehyde + CoA + NAD+ | - |
Citrobacter sp. | acetyl-CoA + NADH | - |
? | |
acetaldehyde + NAD+ | - |
Citrobacter sp. | acetate + NADH + H+ | - |
? | |
butyraldehyde + CoA + NAD+ | 21% of the activity with acetaldehyde | Citrobacter sp. | butanoyl-CoA + NADH | - |
? | |
additional information | bifunctional enzyme consisting of an N-terminal acetaldehyde dehydrogenase (ALDH) and a C-terminal alcohol dehydrogenase (ADH). The specificity constant (kcat/Km) is 47fold higher for acetaldehyde reductase than that for ethanol dehydrogenase | Citrobacter sp. | ? | - |
? | |
propionaldehyde + CoA + NAD+ | 51.8% of the activity with acetaldehyde | Citrobacter sp. | propanoyl-CoA + NADH | - |
? | |
valeraldehyde + CoA + NAD+ | 5.6% of the activity with acetaldehyde | Citrobacter sp. | valeryl-CoA + NADH | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 96000, SDS-PAGE | Citrobacter sp. |
Synonyms | Comment | Organism |
---|---|---|
AdhE | - |
Citrobacter sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Citrobacter sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
inactivation above | Citrobacter sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
13 | - |
acetaldehyde | pH 7.0, 30°C | Citrobacter sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Citrobacter sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
complete inactivation | Citrobacter sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | 57fold preferred over NADP+ | Citrobacter sp. | |
NADP+ | NAD+ is 57fold preferred over NADP+ | Citrobacter sp. |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.3 | - |
acetaldehyde | pH 7.0, 30°C | Citrobacter sp. |