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Literature summary for 1.18.6.1 extracted from

  • Yang, Z.Y.; Dean, D.R.; Seefeldt, L.C.
    Molybdenum nitrogenase catalyzes the reduction and coupling of CO to form hydrocarbons (2011), J. Biol. Chem., 286, 19417-19421.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
synthesis MoFe subunit mutants V70A and V70G will catalyze the reduction and coupling of CO to form methane, ethane, ethylene, propene, and propane. The rates and ratios of hydrocarbon production from CO can be adjusted by changing the flux of electrons through nitrogenase, by substitution of other amino acids located near the FeMo-cofactor, or by changing the partial pressure of CO. Increasing the partial pressure of CO shifts the product ratio in favor of the longer chain alkanes and alkenes Azotobacter vinelandii

Cloned(Commentary)

Cloned (Comment) Organism
-
Azotobacter vinelandii

Protein Variants

Protein Variants Comment Organism
V70A mutation in MoFe subunit. Mutant protein will catalyze the reduction and coupling of CO to form methane, ethane, ethylene, propene, and propane Azotobacter vinelandii
V70G mutation in MoFe subunit. Mutant protein will catalyze the reduction and coupling of CO to form methane, ethane, ethylene, propene, and propane Azotobacter vinelandii

Organism

Organism UniProt Comment Textmining
Azotobacter vinelandii
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-
-
Azotobacter vinelandii DJ1260
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-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CO + dithionite + H+ no substrate for wild-type Azotobacter vinelandii CH4 + C2H6 + C2H4 + C3H6 + C3H8 + ? MoFe subunit mutants V70A and V70G will catalyze the reduction and coupling of CO to form methane, ethane, ethylene, propene, and propane. The rates and ratios of hydrocarbon production from CO can be adjusted by changing the flux of electrons through nitrogenase, by substitution of other amino acids located near the FeMo-cofactor, or by changing the partial pressure of CO. Increasing the partial pressure of CO shifts the product ratio in favor of the longer chain alkanes and alkenes ?
CO + dithionite + H+ no substrate for wild-type Azotobacter vinelandii DJ1260 CH4 + C2H6 + C2H4 + C3H6 + C3H8 + ? MoFe subunit mutants V70A and V70G will catalyze the reduction and coupling of CO to form methane, ethane, ethylene, propene, and propane. The rates and ratios of hydrocarbon production from CO can be adjusted by changing the flux of electrons through nitrogenase, by substitution of other amino acids located near the FeMo-cofactor, or by changing the partial pressure of CO. Increasing the partial pressure of CO shifts the product ratio in favor of the longer chain alkanes and alkenes ?