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Literature summary for 1.18.6.1 extracted from

  • Shah, V.K.; Ugalde, R.A.; Imperial, J.; Brill, W.J.
    Molybdenum in nitrogenase (1984), Annu. Rev. Biochem., 53, 231-257.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Azotobacter vinelandii

Inhibitors

Inhibitors Comment Organism Structure
CO
-
Azotobacter vinelandii
CO
-
Clostridium pasteurianum
CO
-
Klebsiella pneumoniae
CO
-
Paenibacillus polymyxa
O2
-
Azotobacter vinelandii
O2
-
Clostridium pasteurianum
O2
-
Klebsiella pneumoniae
O2
-
Paenibacillus polymyxa

Metals/Ions

Metals/Ions Comment Organism Structure
Iron
-
Clostridium pasteurianum
Iron
-
Paenibacillus polymyxa
Iron enzyme consists of 2 proteins: a molybdenum and iron-containing protein, MoFe protein, component I, dinitrogenase, and an iron containing protein, Fe protein, component II, dinitrogenase reductase, together they form the active nitrogenase complex Klebsiella pneumoniae
Iron enzyme consists of 2 proteins: a molybdenum and iron-containing protein, MoFe protein, component I, dinitrogenase, and an iron containing protein, Fe protein, component II, dinitrogenase reductase, together they form the active nitrogenase complex Azotobacter vinelandii
Iron 4 atoms of iron per molecule of Fe protein Klebsiella pneumoniae
Iron 4 atoms of iron per molecule of Fe protein Azotobacter vinelandii
Mg2+ Mg2+ required for MgATP complex Klebsiella pneumoniae
Mg2+ Mg2+ required for MgATP complex Clostridium pasteurianum
Mg2+ Mg2+ required for MgATP complex Azotobacter vinelandii
Mg2+ Mg2+ required for MgATP complex Paenibacillus polymyxa
Molybdenum review on molybdenum in nitrogenase Klebsiella pneumoniae
Molybdenum review on molybdenum in nitrogenase Clostridium pasteurianum
Molybdenum review on molybdenum in nitrogenase Azotobacter vinelandii
Molybdenum review on molybdenum in nitrogenase Paenibacillus polymyxa
Molybdenum 6 iron atoms to 1 molybdenum atom in MoFe protein Klebsiella pneumoniae
Molybdenum molybdenum metabolism, cofactor synthesis from nif genes , regulation and structure, overview Klebsiella pneumoniae
Molybdenum molybdenum metabolism, cofactor synthesis from nif genes , regulation and structure, overview Clostridium pasteurianum
Molybdenum molybdenum metabolism, cofactor synthesis from nif genes , regulation and structure, overview Azotobacter vinelandii
Molybdenum enzyme consists of 2 proteins: a molybdenum and iron-containing protein (MoFe protein, component I, dinitrogenase) and an iron containing protein (Fe protein, component II, dinitrogenase reductase), together they form the active nitrogenase complex Klebsiella pneumoniae
Molybdenum enzyme consists of 2 proteins: a molybdenum and iron-containing protein (MoFe protein, component I, dinitrogenase) and an iron containing protein (Fe protein, component II, dinitrogenase reductase), together they form the active nitrogenase complex Azotobacter vinelandii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000 60000 component II Fe protein Klebsiella pneumoniae
55000 60000 component II Fe protein Clostridium pasteurianum
55000 60000 component II Fe protein Azotobacter vinelandii
220000 250000 component I MoFe protein Klebsiella pneumoniae
220000 250000 component I MoFe protein Clostridium pasteurianum
220000 250000 component I MoFe protein Azotobacter vinelandii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O Klebsiella pneumoniae
-
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
?
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O Clostridium pasteurianum
-
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
?
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O Azotobacter vinelandii
-
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
?
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O Paenibacillus polymyxa
-
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
?

Organism

Organism UniProt Comment Textmining
Azotobacter vinelandii
-
-
-
Clostridium pasteurianum
-
-
-
Klebsiella pneumoniae
-
nitrogen fixation complex is encoded on nif gene cluster
-
Paenibacillus polymyxa
-
-
-

Oxidation Stability

Oxidation Stability Organism
MoFe protein is extremely sensitive to O2 Klebsiella pneumoniae
MoFe protein is extremely sensitive to O2 Azotobacter vinelandii

Reaction

Reaction Comment Organism Reaction ID
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate catalytic mechanism Klebsiella pneumoniae
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate catalytic mechanism Clostridium pasteurianum
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate catalytic mechanism Azotobacter vinelandii
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate catalytic mechanism Paenibacillus polymyxa
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein Klebsiella pneumoniae
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein Clostridium pasteurianum
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein Azotobacter vinelandii
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein Paenibacillus polymyxa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
Klebsiella pneumoniae 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
?
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
Clostridium pasteurianum 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
?
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
Azotobacter vinelandii 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
?
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
Paenibacillus polymyxa 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
?