Crystallization (Comment) | Organism |
---|---|
- |
Azotobacter vinelandii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CO | - |
Azotobacter vinelandii | |
CO | - |
Clostridium pasteurianum | |
CO | - |
Klebsiella pneumoniae | |
CO | - |
Paenibacillus polymyxa | |
O2 | - |
Azotobacter vinelandii | |
O2 | - |
Clostridium pasteurianum | |
O2 | - |
Klebsiella pneumoniae | |
O2 | - |
Paenibacillus polymyxa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | - |
Clostridium pasteurianum | |
Iron | - |
Paenibacillus polymyxa | |
Iron | enzyme consists of 2 proteins: a molybdenum and iron-containing protein, MoFe protein, component I, dinitrogenase, and an iron containing protein, Fe protein, component II, dinitrogenase reductase, together they form the active nitrogenase complex | Klebsiella pneumoniae | |
Iron | enzyme consists of 2 proteins: a molybdenum and iron-containing protein, MoFe protein, component I, dinitrogenase, and an iron containing protein, Fe protein, component II, dinitrogenase reductase, together they form the active nitrogenase complex | Azotobacter vinelandii | |
Iron | 4 atoms of iron per molecule of Fe protein | Klebsiella pneumoniae | |
Iron | 4 atoms of iron per molecule of Fe protein | Azotobacter vinelandii | |
Mg2+ | Mg2+ required for MgATP complex | Klebsiella pneumoniae | |
Mg2+ | Mg2+ required for MgATP complex | Clostridium pasteurianum | |
Mg2+ | Mg2+ required for MgATP complex | Azotobacter vinelandii | |
Mg2+ | Mg2+ required for MgATP complex | Paenibacillus polymyxa | |
Molybdenum | review on molybdenum in nitrogenase | Klebsiella pneumoniae | |
Molybdenum | review on molybdenum in nitrogenase | Clostridium pasteurianum | |
Molybdenum | review on molybdenum in nitrogenase | Azotobacter vinelandii | |
Molybdenum | review on molybdenum in nitrogenase | Paenibacillus polymyxa | |
Molybdenum | 6 iron atoms to 1 molybdenum atom in MoFe protein | Klebsiella pneumoniae | |
Molybdenum | molybdenum metabolism, cofactor synthesis from nif genes , regulation and structure, overview | Klebsiella pneumoniae | |
Molybdenum | molybdenum metabolism, cofactor synthesis from nif genes , regulation and structure, overview | Clostridium pasteurianum | |
Molybdenum | molybdenum metabolism, cofactor synthesis from nif genes , regulation and structure, overview | Azotobacter vinelandii | |
Molybdenum | enzyme consists of 2 proteins: a molybdenum and iron-containing protein (MoFe protein, component I, dinitrogenase) and an iron containing protein (Fe protein, component II, dinitrogenase reductase), together they form the active nitrogenase complex | Klebsiella pneumoniae | |
Molybdenum | enzyme consists of 2 proteins: a molybdenum and iron-containing protein (MoFe protein, component I, dinitrogenase) and an iron containing protein (Fe protein, component II, dinitrogenase reductase), together they form the active nitrogenase complex | Azotobacter vinelandii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | 60000 | component II Fe protein | Klebsiella pneumoniae |
55000 | 60000 | component II Fe protein | Clostridium pasteurianum |
55000 | 60000 | component II Fe protein | Azotobacter vinelandii |
220000 | 250000 | component I MoFe protein | Klebsiella pneumoniae |
220000 | 250000 | component I MoFe protein | Clostridium pasteurianum |
220000 | 250000 | component I MoFe protein | Azotobacter vinelandii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O | Klebsiella pneumoniae | - |
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate | - |
? | |
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O | Clostridium pasteurianum | - |
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate | - |
? | |
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O | Azotobacter vinelandii | - |
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate | - |
? | |
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O | Paenibacillus polymyxa | - |
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Azotobacter vinelandii | - |
- |
- |
Clostridium pasteurianum | - |
- |
- |
Klebsiella pneumoniae | - |
nitrogen fixation complex is encoded on nif gene cluster | - |
Paenibacillus polymyxa | - |
- |
- |
Oxidation Stability | Organism |
---|---|
MoFe protein is extremely sensitive to O2 | Klebsiella pneumoniae |
MoFe protein is extremely sensitive to O2 | Azotobacter vinelandii |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate | catalytic mechanism | Klebsiella pneumoniae | |
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate | catalytic mechanism | Clostridium pasteurianum | |
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate | catalytic mechanism | Azotobacter vinelandii | |
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate | catalytic mechanism | Paenibacillus polymyxa | |
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate | enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein | Klebsiella pneumoniae | |
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate | enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein | Clostridium pasteurianum | |
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate | enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein | Azotobacter vinelandii | |
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate | enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein | Paenibacillus polymyxa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O | - |
Klebsiella pneumoniae | 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate | - |
? | |
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O | - |
Clostridium pasteurianum | 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate | - |
? | |
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O | - |
Azotobacter vinelandii | 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate | - |
? | |
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O | - |
Paenibacillus polymyxa | 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate | - |
? |