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Literature summary for 1.18.1.2 extracted from

  • Morsy, F.; Nakajima, M.; Yoshida, T.; Fujiwara, T.; Sakamoto, T.; Wada, K.
    Subcellular localization of ferredoxin-NADP+ oxidoreductase in phycobilisome retaining oxygenic photosysnthetic organisms (2008), Photosynth. Res., 95, 73-85.
    View publication on PubMed
Search for more literature for 1.18.1.2

Activating Compound

Activating Compound Comment Organism Structure
NaCl quinone-dependent activity decreases at concentration below 0.1 M and over 0.5 M Arthrospira platensis
NaCl quinone-dependent activity decreases at concentrations below 0.1 M and over 0.7 M Cyanidium caldarium

Inhibitors

Inhibitors Comment Organism Structure
NaCl ferredoxin-dependent activity decreases with increasing NaCl concentration and disappears at concentrations over 0.5 M NaCl Arthrospira platensis
NaCl ferredoxin-dependent activity decreases with increasing NaCl concentration and disappears at concentrations over 0.5 M NaCl Cyanidium caldarium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0011
-
 NADPH in a quinone-dependent cytochrome c reduction assay Arthrospira platensis
0.0011
-
 NADPH in a quinone-dependent cytochrome c reduction assay Cyanidium caldarium
0.004
-
 NADPH in a ferredoxin-dependent cytochrome c reduction assay Arthrospira platensis
0.004
-
 NADPH in a ferredoxin-dependent cytochrome c reduction assay Cyanidium caldarium
0.011
-
 NADPH in a 2,6-dichlorophenolindophenol reduction assay Arthrospira platensis
0.02
-
 NADPH in a 2,6-dichlorophenolindophenol reduction assay Cyanidium caldarium

Localization

Localization Comment Organism GeneOntology No. Textmining
phycobilisome
-
 Arthrospira platensis 30089
-
thylakoid membrane
-
 Arthrospira platensis 42651
-
thylakoid membrane
-
 Cyanidium caldarium 42651
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
 SDS-PAGE Cyanidium caldarium
34000
-
 gel filtration Cyanidium caldarium
46000
-
 SDS-PAGE Arthrospira platensis
47000
-
 gel filtration Arthrospira platensis
90000
-
 NaCl solubilized FNR from Spirulina platensis thylakoid membranes contained the 46000 Da FNR and a 17000 Da protein Arthrospira platensis

Organism

Organism UniProt Comment Textmining
Arthrospira platensis
-
 cyanobacterium
-
Arthrospira platensis IAM M-135
-
 cyanobacterium
-
Cyanidium caldarium A0ZSY5 red alga
-
Cyanidium caldarium RK-1 A0ZSY5 red alga
-

Purification (Commentary)

Purification (Comment) Organism
-
 Arthrospira platensis
-
 Cyanidium caldarium

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
25
-
 ferredoxin-dependent activity at pH 9.5 Cyanidium caldarium
30
-
 ferredoxin-dependent activity at pH 10 Arthrospira platensis
60
-
 quinone-dependent activity at pH 7.5 Arthrospira platensis
60
-
 quinone-dependent activity at pH 7.5 Cyanidium caldarium
70
-
 ferredoxin-dependent activity at pH 6.5 Arthrospira platensis
77
-
-
 Cyanidium caldarium
85
-
-
 Arthrospira platensis
95
-
 ferredoxin-dependent activity at pH 7 Arthrospira platensis
110
-
 ferredoxin-dependent activity at pH 6.5 Cyanidium caldarium
140
-
 ferredoxin-dependent activity at pH 7 Cyanidium caldarium
260
-
 quinone-dependent activity at pH 10 Arthrospira platensis
280
-
 quinone-dependent activity at pH 9.7 Arthrospira platensis
325
-
 quinone-dependent activity at pH 10 Cyanidium caldarium
400
-
 quinone-dependent activity at pH 9.7 Cyanidium caldarium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NADPH + H+ + oxidized 2,6-dichlorophenolindophenol
-
 Arthrospira platensis NADP+ + reduced 2,6-dichlorophenolindophenol
-
 ?
NADPH + H+ + oxidized 2,6-dichlorophenolindophenol
-
 Cyanidium caldarium NADP+ + reduced 2,6-dichlorophenolindophenol
-
 ?
NADPH + H+ + oxidized 2,6-dichlorophenolindophenol
-
 Arthrospira platensis IAM M-135 NADP+ + reduced 2,6-dichlorophenolindophenol
-
 ?
NADPH + H+ + oxidized 2,6-dichlorophenolindophenol
-
 Cyanidium caldarium RK-1 NADP+ + reduced 2,6-dichlorophenolindophenol
-
 ?
oxidized ferredoxin + NADPH + cytochrome c
-
 Arthrospira platensis reduced ferredoxin + NADP+ + reduced cytochrome c
-
 ?
oxidized ferredoxin + NADPH + cytochrome c
-
 Cyanidium caldarium reduced ferredoxin + NADP+ + reduced cytochrome c
-
 ?
oxidized ferredoxin + NADPH + cytochrome c
-
 Arthrospira platensis IAM M-135 reduced ferredoxin + NADP+ + reduced cytochrome c
-
 ?
oxidized ferredoxin + NADPH + cytochrome c
-
 Cyanidium caldarium RK-1 reduced ferredoxin + NADP+ + reduced cytochrome c
-
 ?
phenyl-p-benzoquinone + NADPH + cytochrome c
-
 Arthrospira platensis ? + NADP+ + reduced cytochrome c
-
 ?
phenyl-p-benzoquinone + NADPH + cytochrome c
-
 Cyanidium caldarium ? + NADP+ + reduced cytochrome c
-
 ?
phenyl-p-benzoquinone + NADPH + cytochrome c
-
 Arthrospira platensis IAM M-135 ? + NADP+ + reduced cytochrome c
-
 ?
phenyl-p-benzoquinone + NADPH + cytochrome c
-
 Cyanidium caldarium RK-1 ? + NADP+ + reduced cytochrome c
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 34000, gel filtration Cyanidium caldarium
monomer 1 * 47000, gel filtration Arthrospira platensis

Synonyms

Synonyms Comment Organism
ferredoxin-NADP+ oxidoreductase
-
 Arthrospira platensis
ferredoxin-NADP+ oxidoreductase
-
 Cyanidium caldarium
FNR
-
 Arthrospira platensis
FNR
-
 Cyanidium caldarium

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 in a 2,6-dichlorophenolindophenol reduction assay Arthrospira platensis
40
-
 in a ferredoxin-dependent cytochrome c reduction assay Arthrospira platensis
45
-
 in a quinone-dependent cytochrome c reduction assay Arthrospira platensis
50
-
 in a ferredoxin-dependent cytochrome c reduction assay Cyanidium caldarium
55
-
 in a 2,6-dichlorophenolindophenol reduction assay Cyanidium caldarium
55
-
 in a quinone-dependent cytochrome c reduction assay Cyanidium caldarium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 in a ferredoxin-dependent cytochrome c reduction assay Arthrospira platensis
7
-
 in a ferredoxin-dependent cytochrome c reduction assay Cyanidium caldarium
9.7
-
 in a 2,6-dichlorophenolindophenol reduction assay Arthrospira platensis
9.7
-
 in a 2,6-dichlorophenolindophenol reduction assay Cyanidium caldarium
9.7
-
 in a quinone-dependent cytochrome c reduction assay Arthrospira platensis
9.7
-
 in a quinone-dependent cytochrome c reduction assay Cyanidium caldarium

pH Range

pH Minimum pH Maximum Comment Organism
6.5 10 ferredoxin-dependent activity at pH 6.5 is 70 micromol/min/mg, at pH 7 is 95 micromol/min/mg and at pH 10 is 30 micromol/min/mg Arthrospira platensis
6.5 9.5 ferredoxin-dependent activity at pH 6.5 is 110 micromol/min/mg, at pH 7 is 140 micromol/min/mg and at pH 9.5is 25 micromol/min/mg Cyanidium caldarium
7.5 10 quinone-dependent activity at pH 7.5 is 60 micromol/min/mg, at pH 9.7 is 280 micromol/min/mg and at pH 10 is 260 micromol/min/mg Arthrospira platensis
7.5 10 quinone-dependent activity at pH 7.5 is 60 micromol/min/mg, at pH 9.7 is 400 micromol/min/mg and at pH 10 is 325 micromol/min/mg Cyanidium caldarium