Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as recombinant protein | Hydrogenobacter thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Fpr showed high specificity for NADPH; Km value for NADPH is <5 microMol, whereas that for NADH is above 2 mM | Hydrogenobacter thermophilus | |
0.131 | - |
NADP+ | - |
Hydrogenobacter thermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24600 | - |
gel filtration | Hydrogenobacter thermophilus |
30000 | - |
SDS-PAGE | Hydrogenobacter thermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
oxidized ferredoxin + NADPH | Hydrogenobacter thermophilus | Hydrogenobacter thermophilus expresses three ferredoxins: [4Fe-4S]-type Fd1 and Fd2, and [2Fe-2S]-type Fd3 | reduced ferredoxin + NADP+ | - |
r | |
oxidized ferredoxin + NADPH | Hydrogenobacter thermophilus TK-6 / IAM 12695 | Hydrogenobacter thermophilus expresses three ferredoxins: [4Fe-4S]-type Fd1 and Fd2, and [2Fe-2S]-type Fd3 | reduced ferredoxin + NADP+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hydrogenobacter thermophilus | - |
- |
- |
Hydrogenobacter thermophilus TK-6 / IAM 12695 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Hydrogenobacter thermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
21.2 | - |
Vmax value is 28 micromol/min/mg when NADPH is used as the electron donor, Vmax value is 22 micromol/min/mg when reduced ferredoxin Fd1 is used as the electron donor and NADP+ as electron acceptor | Hydrogenobacter thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADPH + H+ + oxidized 2,6-dichlorophenolindophenol | - |
Hydrogenobacter thermophilus | NADP+ + reduced 2,6-dichlorophenolindophenol | - |
r | |
NADPH + H+ + oxidized 2,6-dichlorophenolindophenol | - |
Hydrogenobacter thermophilus TK-6 / IAM 12695 | NADP+ + reduced 2,6-dichlorophenolindophenol | - |
r | |
oxidized ferredoxin + NADPH | - |
Hydrogenobacter thermophilus | reduced ferredoxin + NADP+ | - |
r | |
oxidized ferredoxin + NADPH | Hydrogenobacter thermophilus expresses three ferredoxins: [4Fe-4S]-type Fd1 and Fd2, and [2Fe-2S]-type Fd3 | Hydrogenobacter thermophilus | reduced ferredoxin + NADP+ | - |
r | |
oxidized ferredoxin + NADPH | - |
Hydrogenobacter thermophilus TK-6 / IAM 12695 | reduced ferredoxin + NADP+ | - |
r | |
oxidized ferredoxin + NADPH | Hydrogenobacter thermophilus expresses three ferredoxins: [4Fe-4S]-type Fd1 and Fd2, and [2Fe-2S]-type Fd3 | Hydrogenobacter thermophilus TK-6 / IAM 12695 | reduced ferredoxin + NADP+ | - |
r | |
reduced ferredoxin + NADP+ | reaction is performed in presence of CoA | Hydrogenobacter thermophilus | oxidized ferredoxin + NADPH | - |
r | |
reduced ferredoxin + NADP+ | reaction is performed in presence of CoA | Hydrogenobacter thermophilus TK-6 / IAM 12695 | oxidized ferredoxin + NADPH | - |
r |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 24600, gel filtration | Hydrogenobacter thermophilus |
Synonyms | Comment | Organism |
---|---|---|
ferredoxin-NADP+ reductase | - |
Hydrogenobacter thermophilus |
FNR | - |
Hydrogenobacter thermophilus |
FPR | - |
Hydrogenobacter thermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | protein contains flavin mononucleotide instead of FAD | Hydrogenobacter thermophilus |